Study the pattern of gene expression in melanoma of pregnant mouse treated with Viscum album

• Grant number: 10/15938-6
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2010
• End date: November 30, 2012
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Mary Uchiyama Nakamura
• Grantee: Mary Uchiyama Nakamura
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Anamaria da Silva Facina ; Fernando Augusto de Almeida ; Ismael Dale Cotrim Guerreiro da Silva

Abstract

The incidence of melanoma is increasing worldwide. It is one of the leading cancers in pregnancy and the most common malignancy to metastasize to placenta and fetus. Viscum album (VA) preparations consist of aqueous extracts of different types of lectins of mistletoe. VA exert cytotoxic and immunomodulatory properties that may be relevant for the inhibition of tumor growth. We are interested in the effects of VA preparation (VA Qu FrF) on growth of B16F10 melanoma implanted in mouse. We propose to study in the experimental model, C57BL6 mice using cells B16-F10 melanoma, the variation of gene expression in melanoma during pregnancy as well as evaluating the antitumor properties of Viscum album. Aim:1-evaluate the expressions of genes in melanoma during pregnancy and beyond through the PCRarray method to study apoptotic cells death pathway. 2- evaluate the expressions of genes in this pathway in melonoma with the use of Viscum Album by PCR method array. Methodo: 1- group - consisting of six pregnant female mouses with implanted tumor cells in the subcutaneous tissue and PBS aroud tumor for 19 days as controls. 2- group - consisting of six non-pregnant female mouses with were implanted with tumor cells subcutaneous, and PBS subcutaneous aroud tumor for 19 as control. 3- non-pregnant group - consisting of six non-pregnant female mouse with implanted tumor cells via subcutaneous, who will receive Viscum album subcutaneous around tumor for 19 days. 4 - Group - consisting of six pregnant female mice with implanted tumor cells via subcutaneous, who receive Viscum album subcutaneous around tumor for 19 days. This method will be used in our study to determine the relative expression levels of genes of interest. (AU)