Morphologic evaluation and comparison between types of muscle fibers of the lateral and medial gastrocnemius and flexor halucis longus after painful stimulation in the neonatal period

• Grant number: 10/12518-6
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2011
• End date: February 28, 2013
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Valéria Paula Sassoli Fazan
• Grantee: Valéria Paula Sassoli Fazan
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

This study aims to evaluate and compare the muscle fibers of the lateral gastrocnemius muscle, medial gastrocnemius (bilateral) in rats, males and females exposed to painful stimuli after birth, at different stages of development. For the study, will be used a control group of 48 animals (6 females and 6 males with 15 days old, 6 females and 6 males with 30 days old, 6 females and 6 males at 90 days old, 6 females 6 males with 180 days of life) and a group of 48 animals to be subjected to painful procedures in the neonatal period (6 females and 6 males with 15 days old, 6 females and 6 males with 30 days old, 6 females and 6 males with 90 days old, 6 females and 6 males with 180 days of life). Pain assessment is performed using the paw pressure and tactile stimulation, assessment of motor function will be carried out using the Functional Assessment of Tibial Nerve. This evaluation will take place every 15 days, during all experimental. After the last functional test, the animals are weighed, anesthetized and perfused with a solution of buffered saline solution followed by Karnowisky. Lateral gastrocnemius muscles and bilateral medial be dissected, prepared with histological techniques for immunostaining of muscle fiber types and cross-sectioned for analysis at the level of light microscopy. The statistical analysis will be performed by specific statistical tests. Considered to be significant differences when p <0.05. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANADA, LUCIANA SAYURI; TAVARES, MARCELO RODRIGO; MAIA NEUBERN, MILENA CARDOSO; SALGADO, HELIO CESAR; SASSOLI FAZAN, VALERIA PAULA. Ratos Wistar podem ser utilizados como os controles normotensos dos ratos espontaneamente hipertensos (SHR) em investigações morfométricas de nervos periféricos?. Acta Cirurgica Brasileira, v. 26, n. 6, p. 514-520, NOV-DEC 2011. (09/16748-9, 09/50389-6, 10/12518-6)

OLIVEIRA, FABRICIO S.; NESSLER, RANDY A.; CASTANIA, JACI A.; SALGADO, HELIO C.; FAZAN, VALERIA P. S.. Ultrastructural and morphometric alterations in the aortic depressor nerve of rats due to long term experimental diabetes: Effects of insulin treatment. Brain Research, v. 1491, p. 197-203, JAN 23 2013. (10/12518-6, 09/50389-6, 09/16748-9)

SANADA, LUCIANA SAYURI; DA ROCHA KALIL, ANDREA LURDES; TAVARES, MARCELO RODRIGO; MAIA NEUBERN, MILENA CARDOSO; SALGADO, HELIO CESAR; SASSOLI FAZAN, VALERIA PAULA. Sural nerve involvement in experimental hypertension: morphology and morphometry in male and female normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive rats (SHR). BMC NEUROSCIENCE, v. 13, MAR 2 2012. (10/12518-6, 09/50389-6, 09/16748-9)

DA COSTA BILEGO NETO, ANTONIO PAULO; CASSIANO SILVEIRA, FERNANDO BRAGA; RODRIGUES DA SILVA, GREICE ANNE; SANADA, LUCIANA SAYURI; SASSOLI FAZAN, VALERIA PAULA. Reproducibility in Nerve Morphometry: Comparison between Methods and among Observers. BIOMED RESEARCH INTERNATIONAL, 2013. (10/12518-6, 09/50389-6)

SATO, KARINA LAURENTI; SANADA, LUCIANA SAYURI; FERREIRA, RENATA DA SILVA; DE BARROS OLIVETI DE MARCO, MARIA CAROLINA DEL BEM; CASTANIA, JACI AIRTON; SALGADO, HELIO CESAR; NESSLER, RANDY ALAN; SASSOLI FAZAN, VALERIA PAULA. Renal nerve ultrastructural alterations in short term and long term experimental diabetes. BMC NEUROSCIENCE, v. 15, JAN 5 2014. (10/12518-6, 09/50389-6, 09/16748-9)

DA COSTA BILEGO NETO, ANTONIO PAULO; CASSIANO SILVEIRA, FERNANDO BRAGA; RODRIGUES DA SILVA, GREICE ANNE; SANADA, LUCIANA SAYURI; SASSOLI FAZAN, VALERIA PAULA. Reproducibility in Nerve Morphometry: Comparison between Methods and among Observers. BIOMED RESEARCH INTERNATIONAL, v. 2013, p. 7-pg., 2013-01-01. (10/12518-6, 09/50389-6)