Neovaginoplasty using oxidized cellulose: genomic analysis of the newly formed epithelium

• Grant number: 10/52721-5
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2011
• End date: June 30, 2014
• Field of knowledge: Biological Sciences - Immunology - Immunogenetics
• Principal Investigator: Zsuzsanna Ilona Katalin de Jármy Di Bella
• Grantee: Zsuzsanna Ilona Katalin de Jármy Di Bella
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Failures in the fusion and tunneling of Müllerian's structures generate malformations of the female internal genitalia. The estimate is approximately 300 new cases per year in our country. Seems that this is an autosomal dominant trait with incomplete penetrance and variable expression of a mutant gene or a chromosome variation that is not yet detected in standard karyotypes. Mullerian anomalies have no corrective treatment, but there are bloodless and surgical methods for the purpose of creating an artificial vagina between the rectum and urethra, although there is no consensus on the best technique. Many tissues can be used in order to coating the newly formed cavity. The use of skin and amniotic membrane leads to metaplasia of the graft gradually transforming it into the vaginal epithelium. The oxidized cellulose membrane, however, is absorbed and epithelialization probably occurs from the transformation of cells of the open area created between the rectum and bladder. The Intent of this study is to identify genes differentially expressed during the process of epithelization of Neovaginas made with oxidized cellulose. Serial biopsy will be performed in the vagina after surgery, during the process of epithelialization. From this material will be extracted RNA for synthesis of complementary DNA that will be used as mold in the PCR reaction in real time, using the kits for the study of Apoptosis, DNA Damage, Angiogenesis, Extracellular Matrix, Growth Factors and Inflammation. This method is used in our study to determine the relative expression levels of genes of interest. (AU)