Acquisition of a microdissection system for the isolation of cells, organelle components, and organisms

• Grant number: 09/54026-5
• Support Opportunities: Multi-user Equipment Program
• Start date: August 01, 2010
• End date: December 31, 2012
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Renee Laufer Amorim
• Grantee: Renee Laufer Amorim
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The molecular characterization of altered cells and tissues has revolutionized the investigation and diagnosis of numerous diseases in humans and in animals. However, the heterogeneity inherent to abnormal primary tissues, because of the coexistence of various populations of reactive cells, can affect the results and the interpretation of the data obtained through various types of analyses. In recent years, the microdissection of histological sections or of cytological preparations has frequently been employed as an alternative for overcoming the obstacles imposed by the complexity of the tissues and for obtaining homogeneous populations of morphologically identified cells. Because of the ever-increasing sensitivity of molecular biology techniques (such as quantitative reverse-transcription polymerase chain reaction in realtime - real-time qRT-PCR - and microarray hybridization analysis), microdissection has become an indispensable tool in pathology research. The petitioning group is composed of 13 researchers active in diverse areas, such as veterinary pathology, clinical veterinary practice, veterinary surgery, genetics (basic and applied), morphology, the study of genetic markers, and molecular biology - four pathologists, four morphologists, two researchers working in clinical/surgical veterinary practice, and three molecular biologists. The composition of the group guarantees that the equipment requested will be used appropriately in all phases of the process, from the morphological characterization and microdissection of the samples to the analyses of gene expression, detection of mutations and epigenetic alterations, as well as being employed at various campuses and extensions of UNESP at São José do Rio Preto (Institute of Biosciences, Literature and Exact sciences) and of UNESP at Botucatu (Biosciences Institute; Department of Genetics; Department of Morphology; School of Veterinary Medicine and Animal Sciences; School of Veterinary and Agricultural Sciences; Department of Clinical Veterinary Medicine; Department of Veterinary Surgery; and Department of Veterinary Pathology). The group is considering studies involving tissues obtained from various species, including dogs, laboratory animals, humans, and arthropods. (AU)