Acquisition of a confocal microscope with cell microinjector and flow cytometer for the advanced investigation of mechanisms of cell death related to oxidative stress

• Grant number: 09/54130-7
• Support Opportunities: Multi-user Equipment Program
• Start date: November 01, 2010
• End date: October 31, 2012
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Iseli Lourenço Nantes Cardoso
• Grantee: Iseli Lourenço Nantes Cardoso
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
• City of the host institution: Santo André
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Tipo de Equipamento Multiusuário não informado
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

In the present project, we propose the acquisition of a confocal microscope with a microinjection system and a flow cytometer for investigating the molecular mechanisms of cell death related to oxidative stress. The associated projects are related to the following: the investigation of the mechanisms of cell death promoted by hemoproteins, hemopeptides, and porphyrins in the aortic cells of rabbits, focusing on the structure and the redox center of each agent of cell death; the investigation of the mechanisms of cell death promoted by phenothiazins in tumor cells, including its effects on the mitochondrial bioenergy and calcium homeostasis; and the investigation of the effects of copper and zinc imbalance on the generation of reactive species in cell culture, with repercussions for the viability of those cells. In addition, the project will benefit a great number of complementary projects in a wide variety of areas, including a project conducted abroad by Professor Rafael Radi, who is also our collaborator on the hemoprotein/ hemopeptide project. Therefore, the equipment requested will make an enormous contribution to research in the state of São Paulo in terms of the publication of articles, the filing of patents, and the development of human resources. (AU)