Identification of genes differentially expressed in the uterus and vagina of rats treated with isoflavones or estrogen

• Grant number: 10/11898-0
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2011
• End date: September 30, 2013
• Field of knowledge: Biological Sciences - Morphology
• Principal Investigator: José Maria Soares Junior
• Grantee: José Maria Soares Junior
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Edmund Chada Baracat ; Ismael Dale Cotrim Guerreiro da Silva ; Manoel João Batista Castello Girão ; Manuel de Jesus Simões

Abstract

In view of the repercussions caused by the use of isoflavones in menopausal and postmenopausal women, it is desirable to study the genetic and molecular mechanisms related to the action of these hormones in the female genital system and also in the expression of extracellular matrix and oxidative stress. The aim of this study is verify if the mechanism of action of the active principles of isoflavones (genistein) is the same of estrogens, ie which genes are expressed in the uterus and vagina. Methodology: 30 females rats, EPM-1 rats (Rattus norvegicus albinos), two months old, will be divided into 3 groups: Group I - immediately after ovariectomy (control) (n = 10) will receive only the vehicle (propylene glycol), Group II - immediately after ovariectomy will receive Genistein (50 mg) (n = 10) Group III - immediately after ovariectomy will receive (17² estradiol 5 m g / kg) (n = 10). Twenty-four hours after the last administration of drugs or vehicle, the uterus and vagina will be removed and immediately be immersed in liquid nitrogen and kept in a freezer at - 80 ° C to the experiments in biology molecular. The cDNA obtained will be used as template in the real time PCR reaction, using the kits for the study of estrogen receptor signaling, extracellular matrix and oxidative stress. With these results we intend to clarify and to suggest changes in the concentration of these two active ingredients and composition of isoflavones marketed in order to avoid possible undesirable side effects. Reactions are analyzed by statistical methods to own the techniques of molecular biology. (AU)