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Keratinocyte growth factor, interleukin 1 beta, 6, 8, 10, 12 and tumor necrosis factor alpha in burned patients

Grant number: 11/12945-4
Support type:Regular Research Grants
Duration: November 01, 2011 - October 31, 2013
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Alfredo Gragnani Filho
Grantee:Alfredo Gragnani Filho
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Intense inflammatory responses are activated by burns that affect a large total body surface area. Changes in plasma levels of cytokines after burns occur before metabolic abnormalities unsettle the patient. So it may be possible to develop therapeutic interventions that may attenuate the acute inflammatory response by decreasing the expression of these cytokines. The importance of growth factors in the healing process was demonstrated in cultured keratinocytes and fibroblasts. The keratinocyte growth factor (KGF) is a growth factor active in the repair of wounds, being the most potent stimulator of mitotic cells. Objective: To assess the level of keratinocyte growth factor (KGF) and IL-1ß, IL-6, IL-8, IL-10, IL-12 and TNF-± of patients with burns produced by cultured primary dermal fibroblasts and the gene expression. Methods: 10 patients will be include (05 patients in the study group and 05 patients in the control group) admitted to the Burns Care Unit of the Discipline of Plastic Surgery, Federal University of São Paulo (UNIFESP) between 25% and 50% of total body surface area (TBSA), deep second-degree or third degree, with need to perform surgical debridement. The control group will be constituted by patients with less than 5% of TBSA, deep second-degree or third degree, with need to perform surgical debridement. The authors will evaluate the levels of IL-1ß, IL-6, IL-8, IL-10, IL-12p70 and tumor necrosis factor alpha (TNF-±) in samples of the culture media of primary dermal fibroblasts of patients selected using flow cytometry. The level of keratinocyte growth factor (KGF), in the same samples will be evaluated by ELISA. The keratinocyte growth factor (KGF) and TNF-alfa gene expression will be evaluated in the culture of primary dermal fibroblasts from the same patients. The gene expression of KGF and cytokines will be done by qRT-PCR and RT-PCR array. The experiments will be done in duplicate. Expected Results: The IL-1, IL-6, IL-8, IL-10, IL-12, TNF-± and KGF and their gene expression should be altered in patients with burns affecting over 25% of the body.Key words: burns, cytokines, inflammation, keratinocyte growth factor, fibroblasts. (AU)

Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ALVES CORREA DE NORONHA, SILVANA APARECIDA; RIBEIRO DE NORONHA, SAMUEL MARCOS; LANZIANI, LARISSA ELIAS; FERREIRA, LYDIA MASAKO; GRAGNANI, ALFREDO. Innate and adaptive immunity gene expression of human keratinocytes cultured of severe burn injury. Acta Cirurgica Brasileira, v. 29, n. 3, p. 60-67, 2014. Web of Science Citations: 4.
MAC CORNICK, SARITA; ALVES CORREA DE NORONHA, SILVANA APARECIDA; RIBEIRO DE NORONHA, SAMUEL MARCOS; CEZILLO, MARCUS V. B.; FERREIRA, LYDIA MASAKO; GRAGNANI, ALFREDO. Toll like receptors gene expression of human keratinocytes cultured of severe burn injury. Acta Cirurgica Brasileira, v. 29, n. 3, p. 33-38, 2014. Web of Science Citations: 2.
RIBEIRO DE NORONHA, SAMUEL MARCOS; ALVES CORREA DE NORONHA, SILVANA APARECIDA; KLEPP, ANTHONY GUERATTO; IPOLITO, MICHELE ZAMPIERI; FERREIRA, LYDIA MASAKO; GRAGNANI, ALFREDO. Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients. Acta Cirurgica Brasileira, v. 29, n. 1, p. 62-68, 2014. Web of Science Citations: 2.
SAMUEL MARCOS RIBEIRO DE NORONHA; SILVANA APARECIDA ALVES CORRÊA DE NORONHA; ANTHONY GUERATTO KLEPP; MICHELE ZAMPIERI IPOLITO; LYDIA MASAKO FERREIRA; ALFREDO GRAGNANI. Keratinocyte growth factor, interleukins (1 beta, 6, 8, 10, 12), and tumor necrosis factor alpha in culture medium of dermal fibroblast of burned patients. Acta Cirurgica Brasileira, v. 29, p. -, 2014.
SARITA MAC CORNICK; SILVANA APARECIDA ALVES CORRÊA DE NORONHA; SAMUEL MARCOS RIBEIRO DE NORONHA; MARCUS V. B. CEZILLO; LYDIA MASAKO FERREIRA; ALFREDO GRAGNANI. Toll like receptors gene expression of human keratinocytes cultured of severe burn injury. Acta Cirurgica Brasileira, v. 29, p. -, 2014.
SILVANA APARECIDA ALVES CORRÊA DE NORONHA; SAMUEL MARCOS RIBEIRO DE NORONHA; LARISSA ELIAS LANZIANI; LYDIA MASAKO FERREIRA; ALFREDO GRAGNANI. Innate and adaptive immunity gene expression of human keratinocytes cultured of severe burn injury. Acta Cirurgica Brasileira, v. 29, p. -, 2014.
SILVANA APARECIDA ALVES CORRÊA DE NORONHA; SAMUEL MARCOS RIBEIRO DE NORONHA; LARISSA ELIAS LANZIANI; MICHELE ZAMPIERI IPOLITO; LYDIA MASAKO FERREIRA; ALFREDO GRAGNANI. Human beta defensin-4 and keratinocyte growth factor gene expression in cultured keratinocyte and fibroblasts of burned patients. Acta Cirurgica Brasileira, v. 29, p. -, 2014.

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