Postnatal development of gastric mucosa: interaction among feeding, hormones and growth factors

• Grant number: 11/17415-3
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2011
• End date: May 31, 2014
• Field of knowledge: Biological Sciences - Morphology
• Principal Investigator: Patrícia Gama
• Grantee: Patrícia Gama
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The correct development of the gastrointestinal tract depends on proper suckling-weaning transition. In the gastric mucosa, hormones and milk-born peptides regulate cell proliferation, differentiation and death. Our hypothesis is that suckling is one of the main regulatory agents during development, and so, changes in dietary pattern or conditions can alter the action of molecules that control growth. In order to test this idea, this proposal aims to evaluate the effects of fasting and early weaning on the expression, distribution and activity of corticosterone, ghrelin and TGFbeta in the gastric epithelium to ultimately investigate the integration of these elements. On the 3rd week of postnatal development, rats will be submitted to fasting or early weaning and the stomach will be collected at different ages to extract RNA, protein and tissue for RT-PCR, qPCR, microarray, protein multiplex array, immunoblot and immunohistochemistry to detect: the expression of genes and proteins; cell signaling; cell proliferation and differentiation and cell cycle control. Results will be put together according to experiments and statistically analyzed. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OGIAS, DANIELA; RATTES, ISADORA C.; HOSOYA, LARISSA Y. M.; ZULIAN, JULIANA G.; YAN, CHAO YUN IRENE; GAMA, PATRICIA. Neonatal- maternal separation primes zymogenic cells in the rat gastric mucosa through glucocorticoid receptor activity. SCIENTIFIC REPORTS, v. 8, JUN 29 2018. (12/10948-9, 11/17415-3, 14/21449-9, 10/03076-0, 13/18459-0)

OGIAS, D.; RATTES, I.; ZULIAN, J. G.; RIGONATTI, C. A.; GAMA, P.. Can gastric cell differentiation be reprogrammed by early life stress?. MOLECULAR BIOLOGY OF THE CELL, v. 26, p. 2-pg., 2015-01-01. (14/21449-9, 11/17415-3, 12/10948-9)

TELES SILVA, MELISSA; MESQUITA DA SILVA, KETHLEEN; CAMPOS RATTES, ISADORA; MARIA AGOSTINI ZONTA, GIZELA; VASQUES DA COSTA, ALINE; GALVAO FIGUEREDO COSTA, RAQUEL; KAREN CORDEIRO NOGUEIRA, LUDIMILA; OGIAS, DANIELA; GAMA, PATRICIA. Immediate and Late Effects of Early Weaning on Rat Gastric Cell Differentiation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 1, JAN 1 2020. (11/17415-3, 12/10948-9, 14/21449-9, 16/21944-5, 18/24972-5, 18/07782-8)

ZULIAN, JULIANA GUIMARAES; MASSARENTI HOSOYA, LARISSA YUKARI; FIGUEIREDO, PRISCILA MOREIRA; OGIAS, DANIELA; OSAKI, LUCIANA HARUMI; GAMA, PATRICIA. Corticosterone activity during early weaning reprograms molecular markers in rat gastric secretory cells. SCIENTIFIC REPORTS, v. 7, MAR 31 2017. (13/18459-0, 11/17415-3, 14/21449-9, 10/03076-0)

FIORE, ANA P. Z. P.; OSAKI, LUCIANA H.; GAMA, PATRICIA. Transforming Growth Factor beta 1 Increases p27 Levels via Synthesis and Degradation Mechanisms in the Hyperproliferative Gastric Epithelium in Rats. PLoS One, v. 9, n. 7, JUL 7 2014. (08/05393-2, 11/17415-3)

VALENTE RAMOS BITTAR, NATALIA MARTINS; ZULIAN, JULIANA GUIMARAES; OGIAS, DANIELA; GAMA, PATRICIA. Ghrelin and GHS-R in the rat gastric mucosa: Are they involved in regulation of growth during early weaning?. NUTRITION, v. 32, n. 1, p. 101-107, JAN 2016. (09/00272-5, 11/17415-3)

GHIZONI, HELOISA; FIGUEIREDO, PRISCILA MOREIRA; MOISAN, MARIE-PIERRE; OGIAS, DANIELA; OSAKI, LUCIANA HARUMI; GAMA, PATRICIA. Regulation of corticosterone function during early weaning and effects on gastric cell proliferation. NUTRITION, v. 30, n. 3, p. 343-349, MAR 2014. (09/00272-5, 11/17415-3, 10/03059-8)