Contributions to elucidation of B chromosome biological roles using as model Rhammatocerus brasiliensis (Orthoptera) and Haplochromis obliquidens (Teleostei): structure, evolution and histone proteins modification patterns

• Grant number: 11/19481-3
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2012
• End date: December 31, 2013
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Diogo Cavalcanti Cabral de Mello
• Grantee: Diogo Cavalcanti Cabral de Mello
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated researchers: César Martins ; Patricia Pasquali Parise Maltempi ; Rita de Cássia de Moura

Abstract

The B chromosomes occur in about 15% of eukaryotes species, being primarily composed of repeated DNAs and heterochromatic. Some studies have been performed regarding the anlysis of distribution, frequency, inheritance mechanisms, structure and origin of these chromosomes. On the other hand, deeper analysis related to the diversification patters of DNA sequences present in the B chromosome and shared with the A complement have not been extensively performed. Moreover, the possible gene activity of this chromosome has not been accuracy tested. The cichlid fish have been received great scientific interest due some species have passed through an extensive adaptative radiation process. In some species of this group the presence of a B chromosome was described, as observed in Haplochromis obliquidens. Among insects, the grasshoppers, such as Rhammatocerus brasiliensis, are useful models to analysis of structure and evolution of B chromosomes, however the studies still fragmented and frequently restrict to clasical chromosome analysis, except in Eyprepocnemis plorans and Locusta migratoria. The presente project aim to analyse the genomic content, origin, evolution and possible gene activity of the B chromosomes, using as models H. obliquidens e R. Brasiliensis. In these species, besides the clasic chromosomal analysis, molecular studies related to sequences present in the B chromosmes (shared with the A complemente) will be possible through the use of molecular data and tools, such as microdissection, nucleotide sequencing and genomic analysis using bioinformatics; besides the analusis of histone proteins pos-translationl modification patterns. These analysis integrating the physical mapping and molecular data are promissing in the elucidation of a miriad of questions related to B chromosome diversification mechanisms, enabiling a deeper knowledge concerning the possible biological roles of these elements in eukaryotes. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AMORIM, IGOR COSTA; MILANI, DIOGO; CABRAL-DE-MELLO, DIOGO CAVALCANTI; ROCHA, MARILIA FRANCA; MOURA, RITA CASSIA. Possible origin of B chromosome in Dichotomius sericeus (Coleoptera). Genome, v. 59, n. 8, p. 575-580, AUG 2016. (11/19481-3)

PALACIOS-GIMENEZ, OCTAVIO M.; CASTILLO, ELIO R.; MARTI, DARDO A.; CABRAL-DE-MELLO, DIOGO C.. Tracking the evolution of sex chromosome systems in Melanoplinae grasshoppers through chromosomal mapping of repetitive DNA sequences. BMC Evolutionary Biology, v. 13, AUG 9 2013. (12/01421-7, 11/19481-3)

XAVIER, CRISLAINE; CABRAL-DE-MELLO, DIOGO CAVALCANTI; DE MOURA, RITA CASSIA. Heterochromatin and molecular characterization of DsmarMITE transposable element in the beetle Dichotomius schiffleri (Coleoptera: Scarabaeidae). Genetica, v. 142, n. 6, p. 575-581, DEC 2014. (11/19481-3)

BUENO, DANILO; PALACIOS-GIMENEZ, OCTAVIO MANUEL; ANDREA MARTI, DARDO; MARIGUELA, TATIANE CASAGRANDE; CABRAL-DE-MELLO, DIOGO CAVALCANTI. The 5S rDNA in two Abracris grasshoppers (Ommatolampidinae: Acrididae): molecular and chromosomal organization. Molecular Genetics and Genomics, v. 291, n. 4, p. 1607-1613, AUG 2016. (14/11763-8, 14/02038-8, 11/19481-3, 11/18028-3)

AMORIM, IGOR COSTA; MILANI, DIOGO; CABRAL-DE-MELLO, DIOGO CAVALCANTI; ROCHA, MARILIA FRANCA; MOURA, RITA CASSIA. Possible origin of B chromosome in Dichotomius sericeus (Coleoptera). GENOME, v. 59, n. 8, p. 6-pg., 2016-08-01. (11/19481-3)

ANJOS, ALLISON; PALADINI, ANDRESSA; EVANGELISTA, OLIVIA; CABRAL-DE-MELLO, DIOGO C.. Insights into chromosomal evolution of Cicadomorpha using fluorochrome staining and mapping 18S rRNA and H3 histone genes. JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, v. 57, n. 2, p. 314-322, MAY 2019. (11/19481-3, 14/11763-8)

MILANI, DIOGO; PALACIOS-GIMENEZ, OCTAVIO M.; CABRAL-DE-MELLO, DIOGO C.. The U2 snDNA Is a Useful Marker for B Chromosome Detection and Frequency Estimation in the Grasshopper Abracris flavolineata. Cytogenetic and Genome Research, v. 151, n. 1, p. 36-40, 2017. (11/19481-3, 14/11763-8)

MILANI, DIOGO; RAMOS, ERICA; LORETO, VILMA; ANDREA MARTI, DARDO; CARDOSO, ADAUTO LIMA; MARTINEZ DE MORAES, KAREN CRISTIANE; MARTINS, CESAR; CABRAL-DE-MELLO, DIOGO CAVALCANTI. The satellite DNA AflaSAT-1 in the A and B chromosomes of the grasshopper Abracris flavolineata. BMC GENETICS, v. 18, AUG 29 2017. (15/05246-3, 11/19481-3, 15/16661-1)

ANJOS, A.; RUIZ-RUANO, F. J.; CAMACHO, J. P. M.; LORETO, V.; CABRERO, J.; DE SOUZA, M. J.; CABRAL-DE-MELLO, D. C.. U1 snDNA clusters in grasshoppers: chromosomal dynamics and genomic organization. HEREDITY, v. 114, n. 2, p. 207-219, FEB 2015. (11/19481-3)

MENEZES-DE-CARVALHO, NAHANNA ZIMMERMANN; PALACIOS-GIMENEZ, OCTAVIO MANUEL; MILANI, DIOGO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. High similarity of U2 snDNA sequence between A and B chromosomes in the grasshopper Abracris flavolineata. Molecular Genetics and Genomics, v. 290, n. 5, p. 1787-1792, OCT 2015. (14/02038-8, 12/01421-7, 11/19481-3)

PALACIOS-GIMENEZ, OCTAVIO M.; BUENO, DANILO; CABRAL-DE-MELO, DIOGO C.. Chromosomal mapping of two Mariner-like elements in the grasshopper Abracris flavolineata (Orthoptera: Acrididae) reveals enrichment in euchromatin. European Journal of Entomology, v. 111, n. 3, p. 329-334, JUL 14 2014. (12/01421-7, 11/19481-3)

MILANI, DIOGO; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Microsatellite Organization in the Grasshopper Abracris flavolineata (Orthoptera: Acrididae) Revealed by FISH Mapping: Remarkable Spreading in the A and B Chromosomes. PLoS One, v. 9, n. 5, MAY 28 2014. (11/19481-3)

BUENO, DANILO; PALACIOS-GIMENEZ, OCTAVIO MANUEL; CABRAL-DE-MELLO, DIOGO CAVALCANTI. Chromosomal Mapping of Repetitive DNAs in the Grasshopper Abracris flavolineata Reveal Possible Ancestry of the B Chromosome and H3 Histone Spreading. PLoS One, v. 8, n. 6, JUN 27 2013. (11/19481-3, 11/18028-3)