Characterization of SXT element of bacterial isolates obtained from environmental samples

• Grant number: 11/18826-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: January 31, 2014
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Glavur Rogerio Matté
• Grantee: Glavur Rogerio Matté
• Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Antimicrobial resistance has become a worldwide problem. The mechanisms of antibiotic resistance in bacteria are mediated primarily through mobile genetic elements that carry resistance genes. The SXT element, first isolated in an EGM V. cholerae, has contributed to the spread of resistance genes in different bacterial genus. The first reported observation of the SXT element in South America was held at the Laboratory of Public Health of USP, where these elements were observed in two strains of V. fluvialis. Objective. Characterize and map the SXT elements previously observed in V. fluvialis and expand the investigation of the presence of such elements in isolates from different sources obtained from 1994 and belonging to the collection of cultures of the Public Health Laboratory -FSP-USP. Methods. The existing SXT elements in V. fluvialis (isolates FSP286/08 and FSP561/08) will be characterized using the complete genome sequencing. Investigation of the presence of these elements will be carried out in about 800 isolates belonging to a culture collection of the Laboratory. Positive strains will have antibiotics susceptibility profile determined, and presence of resistance genes investigated. Expected results. Comparing the Brazilian variant SXT element with those existing in epidemic strains available in GenBank, and to design specific primers for Brazilian SXT variants from the analysis of these sequences. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE OLIVEIRA VERAS, ADONNEY ALLAN; DA SILVA, MIRIAM LOPES; MEIRELES GOMES, JAQUELINE CONCEICAO; DIAS, LARISSA MARANHAO; GOMES DE SA, PABLO CARACCIOLO; CASTRO ALVES, JORIANNE THYESKA; CASTRO, WENDEL; MIRANDA, FABIO; KAZUO, EHILTON; MARINHO, DIOGO; et al. Draft Genome Sequences of Vibrio fluvialis Strains 560 and 539, Isolated from Environmental Samples. GENOME ANNOUNCEMENTS, v. 3, n. 1, p. 2-pg., 2015-01-01. (11/18826-7)

DE OLIVEIRA VERAS, ADONNEY ALLAN; DA SILVA, MIRIAM LOPES; MEIRELES GOMES, JAQUELINE CONCEICAO; DIAS, LARISSA MARANHAO; GOMES DE SA, PABLO CARACCIOLO; CASTRO ALVES, JORIANNE THYESKA; CASTRO, WENDEL; MIRANDA, FABIO; KAZUO, EHILTON; MARINHO, DIOGO; et al. Draft Genome Sequences of Vibrio fluvialis Strains 560 and 539, Isolated from Environmental Samples. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 1, JAN-FEB 2015. (11/18826-7)

GOMES DE SA, PABLO CARACCIOLO; DA SILVA, MIRIAM LOPES; CARNEIRO, ADRIANA RIBEIRO; MEIRELES GOMES, JAQUELINE CONCEICAO; DIAS, LARISSA MARANHAO; ALVES, JORIANNE THYESKA CASTRO; DE OLIVEIRA VERAS, ADONNEY ALLAN; BARAUNA, RAFAEL AZEVEDO; DAS GRACAS, DIEGO ASSIS; MATTE, MARIA HELENA; et al. Draft Genome Sequence of Non-O1 and Non-O139 Vibrio cholerae Strain VCC19. GENOME ANNOUNCEMENTS, v. 2, n. 6, p. 2-pg., 2014-11-01. (11/18826-7)