The effect of low level laser therapy in the spectra of visible and infrared light, diphasic current, high voltage in culture of Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli strains in vitro

• Grant number: 11/22170-0
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2012
• End date: February 28, 2014
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Rinaldo Roberto de Jesus Guirro
• Grantee: Rinaldo Roberto de Jesus Guirro
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Infections caused by Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli are among the most frequent in hospitals, which are bacteria that most commonly colonize pressure ulcers. The use of physical agents such as low level laser therapy (LLLT), Diphasic Current and High Voltage has been described as adjuvant therapeutic methods to inhibit the growth of microorganisms, however there is no consensus in the best treatment to be used. The purpose of this study is to analyze the influence of the laser, the diphasic current and high voltage on the rate and growth curve of bacteria in vitro in strains of S. aureus ATCC 25923 (Gram +), P. aeruginosa ATCC 27853 (Gram-) and E. coli ATCC 25922 (Gram-). Upon activation of the strains, the cells are suspended in saline and the concentration adjusted to 103 cells ml-1. The crops will be sown in liquid medium and irradiated with the laser at a wavelength of 450, 660, 830 and 904nm, the fluences of 0 (control), 5, 10, 20, 30, and 40J/cm ², stimulated with the diphasic current 3, 6 and 9mA in 15 min and 30 min, stimulated with the high-voltage 100, 200 and 300V in 30 min and 60 min and incubated at 37 °C. The colony-forming units (CFU / mL) will be counted after the irradiation times of 24, 48, 72, 96, 120, 144, 168 hours. The results will be submitted to a linear regression model with mixed effects (random and fixed effects), with p d 0.05. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, ROSANA CAETANO; BRANDINO, HUGO EVANGELISTA; ALVES DE SOUSA, NATANAEL TEIXEIRA; SANTOS, MARCOS FERRACIOLI; MARTINEZ, ROBERTO; DE JESUS GUIRRO, RINALDO ROBERTO. Polarized currents inhibit in vitro growth of bacteria colonizing cutaneous ulcers. WOUND REPAIR AND REGENERATION, v. 23, n. 3, p. 403-411, MAY-JUN 2015. (11/22170-0, 13/02974-2)

ALVES DE SOUSA, NATANAEL TEIXEIRA; SANTOS, MARCOS FERRACIOLI; GOMES, ROSANA CAETANO; BRANDINO, HUGO EVANGELISTA; MARTINEZ, ROBERTO; DE JESUS GUIRRO, RINALDO ROBERTO. Blue Laser Inhibits Bacterial Growth of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Photomedicine and Laser Surgery, v. 33, n. 5, p. 278-282, MAY 1 2015. (11/22170-0, 13/02974-2)

ALVES DE SOUSA, NATANAEL TEIXEIRA; GOMES, ROSANA CAETANO; SANTOS, MARCOS FERRACIOLI; BRANDINO, HUGO EVANGELISTA; MARTINEZ, ROBERTO; DE JESUS GUIRRO, RINALDO ROBERTO. Red and infrared laser therapy inhibits in vitro growth of major bacterial species that commonly colonize skin ulcers. Lasers in Medical Science, v. 31, n. 3, p. 549-556, APR 2016. (11/22170-0, 13/02974-2)