Genic expession of toll like receptors in the brain of dogs with visceral leishmaniosis

• Grant number: 11/24172-0
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2012
• End date: November 30, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Gisele Fabrino Machado
• Grantee: Gisele Fabrino Machado
• Host Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The infection caused by Leishmania spp. results in disease that presents a wide variation in clinical manifestations, both in humans and dogs. These manifestations vary from observation of asymptomatic infected dogs and the observation of widespread disorders that can result in the death of the host. Chronically infected dogs may show neurological deficit, however there are few reports that characterize the lesions and elucidate the pathogenesis of nervous form of the leishmaniasis. Continuing previous projects and with goal of evaluate the brain lesions in dogs with leishmaniasis and investigate the mechanisms involved in this occurrence, we propose to evaluate the expression of toll-like receptors in the nervous tissue of dogs with visceral leishmaniasis through of real time RT-PCR. Fragments of brain and spleen will be obtained of dogs with diagnostic positive for leishmaniasis (positive diagnosis confirmed by parasitological examination - aspiration biopsy of lymph node and real-time PCR) and with symptomatic manifestation. The gene expression of toll-like receptors 2, 4 and 9 will be assess. Finally, will be evaluated the parasite load in the spleen to determine if there is correlation between the profile of receptors and the level of parasites found in the dog. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELO, G. D.; SILVA, J. E. S.; GRANO, F. G.; HOMEM, C. G.; MACHADO, G. F.. Compartmentalized gene expression of toll-like receptors 2, 4 and 9 in the brain and peripheral lymphoid organs during canine visceral leishmaniasis. PARASITE IMMUNOLOGY, v. 36, n. 12, p. 726-731, DEC 2014. (12/10415-0, 11/24172-0)