| Grant number: | 11/22034-9 |
| Support Opportunities: | Regular Research Grants |
| Start date: | July 01, 2012 |
| End date: | June 30, 2014 |
| Field of knowledge: | Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology |
| Principal Investigator: | Flávia de Oliveira |
| Grantee: | Flávia de Oliveira |
| Host Institution: | Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil |
| Associated researchers: | Edson Aparecido Liberti |
Abstract
Thermal injury causes catabolic processes that conduce to generalized muscle weakness. The purpose of this study is to evaluate morphology, genotoxicity and gene expression of some target genes closely related to inflammatory response in sketical muscle far from original scald burn injury. For this purpose, Wistar rats will be distributed into control (CG) and scald burn injury (SBIG) groups. All rats will be euthanized at 4, 7 and 14 days following the injury. Group SBI will be scalded over 45% of the body surface, standardized by body weight. After euthanasia, the gastrocnemius muscle will be submitted to immunohistochemistry analysis; to MyoD protein expression, ultrastructural analysis by means of transmission electron microscope; genotoxicity evaluation through comet assay; and molecular expression of target genes COX-2, iNOS, TNF-alpha using real time polymerase chain reaction (real time PCR). After the understanding of the mechanisms investigated, this research will contribute to the better establishment of effective strategies in the rehabilitation of muscle weakness in large burn injuries. (AU)
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