Galina Polekhina | St Vincent's Institute of Medical Research - Austrália

• Grant number: 98/11973-8
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: February 23, 1999
• End date: March 14, 1999
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Igor Polikarpov
• Grantee: Igor Polikarpov
• Visiting researcher: Galina Polekhina
• Visiting researcher institution: St. Vincent's Institute of Medical Research, Australia
• Host Institution: Associação Brasileira de Tecnologia de Luz Síncrotron (ABTLuS). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil

Abstract

The main aim of this study is to determine crystallographically 3D structures of the human glutathione synthetase and of the Perfringolysin O, a toxin from Clostridium perfringens. These projects are a collaborative effort of The Ian Potter Foundation Protein Crystallography Laboratory at St. Vincent’s Institute of Medical Research in Melbourne and Laboratório Nacional de Luz Síncrotron (LNLS) in Campinas. Both structures should be determined by Isomorphus Replacement method and are in need of the high resolution synchrotron data. Diffraction data collection of the anomalous signal is of a particular interest for these studies. Apart from the interesting structural information, the methodological part of the project – attempt to solve a structure with use of a single isomorphous replacement with optimized anomalous scattering – is of particular interest for LNLS. This technique has been scarcely used so far in Brazil and its improvement will be of a benefit for all protein crystallographic community of the country. Beamtime, necessary for these projects, is being allocated at the Protein Crystallography beamline at LNLS. (AU)