High content screening: integrated solutions for accelerated drug discovery

• Grant number: 09/11218-1
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: October 17, 2009
• End date: October 31, 2009
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Dulce Elena Casarini
• Grantee: Dulce Elena Casarini
• Visiting researcher: Elaine Del Nery
• Visiting researcher institution: Institut Curie, France
• Host Institution: Departamento de Medicina. Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

High Content Screening (HCS) has become an increasingly powerful tool for cell-based target validation, screening, and lead optimization, and has enabled the application of sophisticated assays for large-scale compound screening. We will examine how HCS has evolved into an integrated solution for accelerated drug discovery. We will look at the workflow components of HCS, including assay design, instrumentation for automated fixed-end-point assays and examine the application and benefits of HCS in live cell kinetic analysis. We will also examine advanced BioApplications that produce information on drug responses from cell image data, informatics/bioinformatics solutions and how to deal with data-management issues related to HCS. This course provides you with a foundation in high-content screening and its applications. (AU)