AbEvo - (Antibodies Evolution): intelligent system for the development of antibodies based on the structure of the antigen

• Grant number: 04/08297-3
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: July 01, 2005
• End date: October 31, 2008
• Field of knowledge: Biological Sciences - Immunology - Applied Immunology
• Principal Investigator: Oscar Henrique Pereira Ramos
• Grantee: Oscar Henrique Pereira Ramos
• Company: Cientistas Associados Desenvolvimento Tecnológico Ltda
• City: São Carlos

Abstract

This project proposes the creation of a computational tool for the development and optimization of antibodies (AbEvo) driven by the structure of a given antigen (in silica evolution). Inspired by the successful programs outlined for the planning of medicines, the tool aims at a reduction in cost, in development time and to facilitate the steps in the production of antibodies. In its first phase, the project envisages the the second stage, will be fine tuned and validated. Among the advantages expected from antibodies conceived using this methodology, the most outstanding is the prevention of hypersensitive reactions caused by the structural reduction stemming from the use of single-chain antibodies (scFVs). The other advantage is that the conception of the proposed tool is perfectly suited to the expression of antibodies in bacterian systems which, consequently, should reduce the time and cost of production. The tool can also be useful for the optimization of antibodies, reducing the occurrence of non-specific molecular recognitions by means of the refinement of molecular interactions. The commercial possibilities prospected on the basis of AvEvo include biomedicines, imaging diagnostic markers and development of diagnostic kits, among others. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CASTRO, J. M. A.; OLIVEIRA, T. S.; SILVEIRA, C. R. F.; CAPORRINO, M. C.; RODRIGUEZ, D.; MOURA-DA-SILVA, A. M.; RAMOS, O. H. P.; RUCAVADO, A.; GUTIERREZ, J. M.; MAGALHAES, G. S.; et al. A neutralizing recombinant single chain antibody, scFv, against BaP1, A P-I hemorrhagic metalloproteinase from Bothrops asper snake venom. Toxicon, v. 87, p. 81-91, SEP 2014. (12/01028-3, 04/08297-3)

OZAKI, CHRISTIANE Y.; SILVEIRA, CAIO R. F.; ANDRADE, FERNANDA B.; NEPOMUCENO, ROBERTO; SILVA, ANDERSON; MUNHOZ, DANIELLE D.; YAMAMOTO, BRUNO B.; LUZ, DANIELA; ABREU, PATRICIA A. E.; HORTON, DENISE S. P. Q.; et al. Single Chain Variable Fragments Produced in Escherichia coli against Heat-Labile and Heat-Stable Toxins from Enterotoxigenic E-coli. PLoS One, v. 10, n. 7, JUL 8 2015. (11/12928-2, 10/14659-6, 11/24111-0, 04/08297-3)