Identification of pharmaceutical vehicles to carry inducers of the peripheral nerve regeneration using tubulization and a new method to study nerve regeneration using end-to-side neurorrhaphy

• Grant number: 12/11012-7
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2012
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Amilton Antunes Barreira
• Grantee: Amilton Antunes Barreira
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The regeneration of nerve fibers depends on several factors such as type of injury and repair, age, loss of tissue and type of injured nerve. One such technique is the nerve tubulization, which uses hollow structures as a bridge between the stumps. The tubulization with a silicone tube is used experimentally for research on regenerative factors because it maintains an environment isolated and inert, preventing the entry and exit of large molecules in the tube. Tubulization is ideal for studying the effect of different substances in the process of nerve regeneration. Another method used to repair peripheral nerve is the end-to-side neurorrhaphy. This is the suturing of the distal stump of injured nerve to the lateral surface of a donor nerve. It is an alternative that can be used when the nerve tissue loss is very large, the end-to-end suture or grafting being impracticable. Experimental studies have shown that the end-to-side neurorrhaphy is functional and allows the sprouting of axons from the lateral aspect of the donor nerve to the nerve stump sutured. The possibility of studies of substances and factors that induce regeneration of nerve fibers has been limited in the end-side neurorrhaphy. The use of a tube interposed between the lateral region of the donor nerve fibers and the distal portion of the receiver - tubing end-to-side - which we propose in this study may resolve this issue. This study will also compare the vehicles agarose, collagen type I and Dulbecco's Modified Eagle Medium (DMEM) to induce regeneration of the sciatic nerve of rats, identifying among the vehicles studied, which one is more appropriate and inert to carry substances or factors which induce nerve regeneration. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VÂNIA TOGNON-MIGUEL; ADRIANA HELENA DO NASCIMENTO-ELIAS; MARIA CRISTINA LOPES SCHIAVONI; AMILTON ANTUNES BARREIRA. Histomorfometria das fibras amielínicas do nervo fibular em ratos Wistar. Arquivos de Neuro-Psiquiatria, v. 74, n. 5, p. 367-372, 2016-05-00. (12/11012-7)

TOGNON-MIGUEL, VANIA; NASCIMENTO-ELIAS, ADRIANA H.; SCHIAVONI, MARIA C. L.; BARREIRO, AMILTON A.. Plasticity of Unmyelinated Fibers in a Side-to-end Tubulization Model. PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN, v. 7, n. 1, JAN 2019. (12/11012-7)