Functional aspects of chromatin protein RaHMG-D during the Rhynchosciara americana development

• Grant number: 12/14091-5
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2012
• End date: October 31, 2014
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Fábio Siviero
• Grantee: Fábio Siviero
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Our group has identified a sequence homologous to HMG-D (High Mobility Group D), a member of a family of abundant nuclear proteins in a cDNA library from salivary glands of Rhynchosciara americana built during the last poytenization replication cycle, at which gene amplification also occurs in several chromosomal regions. The fact that the protein encoded by this message can be involved in remodeling of chromatin and in activation of transcription, making it an attractive target for further study, because are not reported in the literature the involvement of this protein in gene amplification or with the structure of polytene chromosomes. Preliminary data indicate that it may be involved in events occurring during larval development of Rhynchosciara americana. This project aims to complement molecular data that the lab already has, as well as provide morphological and functional assays, characterizating the possible role of this gene during development of R. americana. Techniques such as RT-PCR Real-time and silencing by RNAi are standardized for this gene. By producing a polyclonal serum anti-RaHMG-D we intent to characterize by western blots the protein profile in different tissues, and also determine possible post-translational modifications in 2D westerns. Comparisons in histological sections and preparations of chromosomes from normal individuals and RaHMG-D silenced, may elucidate possible functions of this gene. It is also intended to apply different methodological approach of greater coverage and resolution, as the use of transmission electron microscopy associated with the use of antibodies labelled with gold particles, providing morphological resolution of the RaHMG-D occupancy in polytene chromosomes throughout the development. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIMON, CLAUDIO ROBERTO; SIVIERO, FABIO; MONESI, NADIA. Beyond DNA puffs: What can we learn from studying sciarids?. Genesis, v. 54, n. 7, p. 361-378, JUL 2016. (12/14091-5)

SIMON, CLAUDIO ROBERTO; SIVIERO, FABIO; MONESI, NADIA. Beyond DNA puffs: What can we learn from studying sciarids?. GENESIS, v. 54, n. 7, p. 18-pg., 2016-07-01. (12/14091-5)