Severity and time dependance of ultra-structure of cholangiocytes of rats submitted to I/R injury

• Grant number: 12/51126-1
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2013
• End date: September 30, 2015
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Paolo Rogério de Oliveira Salvalaggio
• Grantee: Paolo Rogério de Oliveira Salvalaggio
• Host Institution: Hospital Israelita Albert Einstein. Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil

Abstract

The term ischemia-reperfusion (I/R) injury commonly refers to clinical, histological and molecular manifestations related to an ischemic process immediately followed by a reperfusion event. In liver surgery and transplantation, I/R injury is associated with worse outcomes and higher rate of complications. Clinically, most of the transplant recipients and patients who undergo liver surgery having severe I/R will present with jaundice. It is known that cholangiocytes are directly injured through the I/R process. Although there has been significant progress in the understanding of I/R in hepatocytes, there is a lack of studies of the effects of I/R on cholangiocytes. Specifically, it is unknown how the cholangiocyte injury progresses and whether it is reversible. Herein, we will postulate that the cholangiocyte damage is Progressive and time-dependent of the de I/R injury. In this study, we will assess the severity and time dependence of ultra-structure of cholangiocytes of rats submitted to I/R injury. Initially, we will refine an in vivo model of I/R in rats, followed by isolation of intra-hepatic cholangiocytes with the use of laser micro-dissection microscopy. We will then study the intensity and time sequence of cholangiocyte changes with the utilization of optic and electronic microscopy. Finally, we will verify the sequence and progression of apoptosis by gene expression analysis. In the future, we intend to test therapeutic agents to reduce I/R effects on the cholangiocyte. Understanding the severity and time sequence of the I/R injury are paramount for future clinical application. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALOIA, THIAGO P. A.; COGLIATI, BRUNO; MONTEIRO, JANAINA M.; GOLDBERG, ANNA C. K.; SALVALAGGIO, PAOLO R. DE OLIVEIRA. Recovery of the Cholangiocytes After Ischemia and Reperfusion Injury: Ultra-Structural, Hystological and Molecular Assessment in Rats. JOURNAL OF CLINICAL AND EXPERIMENTAL HEPATOLOGY, v. 8, n. 4, p. 380-389, DEC 2018. (12/51126-1)