Study of dendritic cells, T cells and NK cells subsets in umbilical cord blood transplant recipients

• Grant number: 12/11730-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2013
• End date: April 30, 2015
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Celso Arrais Rodrigues da Silva
• Grantee: Celso Arrais Rodrigues da Silva
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Mihoko Yamamoto

Abstract

Umbilical cord blood transplantation (UCBT) is a valuable alternative stem cell source which has been increasingly used for hematopoietic stem cell transplantation (HSCT). Natural killer cells (NK cells) and dendritic cells (DC) have their own recovery properties, and modifications on these paths may alter transplant outcomes when bone marrow (BM) or peripheral blood (PB) are used as source of stem cells. Aims: 1) To characterize dendritic, T cells and NK cells recovery after UCBT; 2) To evaluate the impact of dendritic cells, T cells and NK cells subpopulations' reconstitution on the main transplant outcomes; 3) To compare the kinetics of reconstitution of dendritic cells, T cells and NK cells between UCBT and mobilized PB HSCT (PBSCT) recipients. Methods: Forty patients undergoing unrelated allogeneic UCBT, 20 patients undergoing BMT, and 20 patients undergoing PBSCT will be enrolled. Serial blood samples from transplanted patients will be collected at specified times after the transplant. Twenty PB samples from normal volunteers will also be analyzed. Dendritic cells, T cells and NK cells subpopulations will be quantified by multiparametric flow cytometry. Patients will be followed for one year and quantification will be correlated to the following transplant outcomes: engraftment, mortality, relapse, progression free survival and GVHD. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, MATHEUS VESCOVI; YAMAMOTO, MIHOKO; SUGANO KIMURA, ELIZA YURICO; RENSI COLTURATO, VERGILIO ANTONIO; DE SOUZA, MAIR PEDRO; MAUAD, MARCOS; IKOMA, MAURA VALERIO; NOVIS, YANA; ROCHA, VANDERSON; GINANI, VALERIA CORTEZ; et al. Low Counts of Plasmacytoid Dendritic Cells after Engraftment Are Associated with High Early Mortality after Allogeneic Stem Cell Transplantation. BIOLOGY OF BLOOD AND MARROW TRANSPLANTATION, v. 21, n. 7, p. 1223-1229, JUL 2015. (12/11730-7)