Prevalence, molecular characterization and interactions of avian mycoplasmas and laryngotracheitis infectious virus in laying hens of Bastos' region

• Grant number: 12/20912-1
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2013
• End date: December 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Marcos Roberto Buim
• Grantee: Marcos Roberto Buim
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Antonio José Piantino Ferreira ; Claudia Del Fava ; Masaio Mizuno Ishizuka

Abstract

Respiratory diseases are the main causes of sanitary problems in commercial eggs production. Multifactorial agents are involved, and mycoplasmas and Infectious Laryngotracheitis virus (ILT) virus are the most important. Bastos is the main region of eggs production in Brazil, with a herd estimated in 20 million heads. The respiratory diseases that affect the layer hens and cause economic losses, the etiology, the differential diagnose and interactions between these pathogens must be studied. This project aims to determine the prevalence of mycoplasmas and ILT virus, differentiating wild strains from vaccine ones, using sanitary surveillance tools, biomolecular techniques and valuating the interaction between infection. The methodology to be used includes Real Time PCR and DNA sequencing, which allow differentiation among strains, as well the histopathology for characterizing microscopically lesions in upper respiratory tract, as larynx and trachea. This study will bring important scientific information that will be directly applied into the egg production chain, to prophylaxis strategies, to valuate efficacy of compulsory measures for controlling avian mycoplasmosis and ILT, as well to propose actualizations in the Official Avian Health Program of São Paulo State. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PARRA, SILVANA SANTANDER; NUNEZ, LUIS; BUIM, MARCOS R.; ASTOLFI-FERREIRA, CLAUDETE S.; PIANTINO FERREIRA, ANTONIO J.. Development of a qPCR for the detection of infectious laryngotracheitis virus (ILTV) based on the gE gene. BRITISH POULTRY SCIENCE, v. 59, n. 4, p. 402-407, 2018. (12/20912-1)