Histological and immunoistochemical analysis of placenta obtained from eutocic and dystocic bitch parturition

• Grant number: 13/09327-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: January 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Nereu Carlos Prestes
• Grantee: Nereu Carlos Prestes
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Ian Martin ; Maria Denise Lopes ; Rodrigo Volpato

Abstract

The recent expansion of knowledge related to basic research in physiology of dogs reproductive has allowed the use of these animals as experimental models for many researchers. For this reason, a better understanding of functional morphology of this species, especially in relation to placentation, placental structures and his mother-fetus relationship is required. The placenta is also regarded as an endocrine organ, producing hormones that aims signaling and maintenance of pregnancy. Bitches are monoestrous animals whose placenta is the type endotheliochorial and zoonary, besides being considered invasive due to its close relation between the maternal and fetal portions. The objective of this work is histological characterization of marginal placental hematoma, the immunolocalization of progesterone receptors and estrogen, and also the possible correlation of these factors with weight of pups in the born, the age of the mother and the eutocic and dystocic parturition frequency. Bitches be distributed into 3 groups (n = 15) according to breed. In Group I will use bitches of breed Bullbras, in Group II will use bitches of breed Pitbull and Group III will use female without defined breed (SRD). The parturition will be classified as eutocic or dystocic, each newborn will be weighed with numeric ID corresponding to your placenta, will be collected three placentas randomly of each dog, with immediate macroscopic analysis, the set of fetal membranes will be weighed on an analytical precision balance and stored in 10% buffered formalin for 24 hours and then will be kept in 70% alcohol, then will be forwarded to the laboratory for histological and immunohistochemical analyzes. (AU)