Influence of chemotherapy drugs in the evolution of experimental periodontitis in rats

• Grant number: 14/11427-8
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2014
• End date: July 31, 2016
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Juliano Milanezi de Almeida
• Grantee: Juliano Milanezi de Almeida
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Edilson Ervolino ; Letícia Helena Theodoro ; Suely Regina Mogami Bomfim ; Valdir Gouveia Garcia

Abstract

The aim of this animal study will be to evaluate by comparative analysis of the biochemically, tomographic, photometrically, histomorphometrically and immunohistochemically study, the influence of chemotherapeutic drugs on the evolution of experimental periodontitis (EP) in rats. Following a table generated by a computer program, 180 male rats will be divided into 6 groups, with 30 animals each. SPE- SS group, also called the sham group. The animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 0.5 ml of saline solution 0.9 % (SS). These animals will not be induced to EP. EP - SS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 0.5 ml of SS. Soon after the first injection, PE will be induced in the lower first right and left molars. SEP - 5FU group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 60 mg / kg and 40 mg / kg of 5-Fluorouracil (5-FU) respectively. These animals will not be induced to EP. EP - 5FU group: animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 60 mg/kg and 40 mg/kg 5-FU respectively. Soon after the first injection, PE will be induced in the lower first right and left molars. SEP - CIS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg of cisplatin (CIS) respectively. These animals will not be induced to EP. EP - CIS group: the animals in this group will receive two intraperitoneal injections with 48-hour interval between them of 5 mg/kg and 2.5 mg/kg CIS respectively. Soon after the first injection, PE will be induced in the lower first right and left molars. EP was induced by the placement of a number 24 cotton thread around the lower first molars. After of 07, 15 and 30 days after the first intraperitoneal injection (SS or chemotherapy) animals will be euthanized using an overdose of thiopental (150mg/kg). Blood will be collected via cardiac puncture for hematology and biochemical analyzes, at the following times: prior to application of chemotherapy or SS and euthanasia periods (7, 15 and 30 days). Blood samples will be divided into two microtubes, one of them for the blood test and the other for the biochemical analysis of AST, ALT, creatinine and urea. The mandibles collected will be divided into two parts and processed according each analyzes. For the micro-computed tomography (Micro-CT) and photometric analysis will be assessed the loss of alveolar bone volume. The contralateral mandibles are used for histomorphometric analysis which will be evaluated and the percentage of bone histology in the furcation area, and immunohistochemical analyzes of biomarkers in the furcation area (RANKL, OPG, TRAP, BAX, PCNA, HIF1±). Quantitative data will be analyzed statistically (p < 0.05) in a specialized computer program (Bioestat 5.0). (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RODRIGUES GUSMAN, DAVID JONATHAN; ERVOLINO, EDILSON; THEODORO, LETICIA HELENA; GARCIA, VALDIR GOUVEIA; HITOMI NAGATA, MARIA JOSE; SENDAO ALVES, BRENO EDSON; DE ARAUJO, NATHALIA JANUARIO; MATHEUS, HENRIQUE RINALDI; DE ALMEIDA, JULIANO MILANEZI. Antineoplastic agents exacerbate periodontal inflammation and aggravate experimental periodontitis. JOURNAL OF CLINICAL PERIODONTOLOGY, v. 46, n. 4, p. 457-469, APR 2019. (14/11427-8)

MATHEUS, HENRIQUE RINALDI; ERVOLINO, EDILSON; RODRIGUES GUSMAN, DAVID JONATHAN; FORIN, LUIZ GUILHERME; PIOVEZAN, BIANCA RAFAELI; DE ALMEIDA, JULIANO MILANEZI. The influence of antineoplastic agents on the peri-implant bone around osseointegrated titanium implants: an in vivo histomorphometric and immunohistochemical study. CLINICAL ORAL INVESTIGATIONS, OCT 2021. (17/11805-0, 14/11427-8)

DE ALMEIDA, JULIANO MILANEZI; ERVOLINO, EDILSON; GUSMAN, DAVID JONATHAN RODRIGUES; FIORIN, LUIZ GUILHERME; ALVES, BRENO EDSON SENDAO; GUASTALDI, FERNANDO POZZI SEMEGHINI; MATHEUS, HENRIQUE RINALDI. ntineoplastic agents aggravate the damages caused by nicotine on the peri-implant bone: an in vivo histomorphometric and immunohistochemical study in rat. CLINICAL ORAL INVESTIGATIONS, v. 26, n. 2, AUG 2021. (17/11805-0, 14/11427-8)

DE ALMEIDA, JULIANO MILANEZI; MARQUES, BIANCA MAYARA; NORONHA NOVAES, VIVIAN CRISTINA; PINTO DE OLIVEIRA, FRED LUCAS; MATHEUS, HENRIQUE RINALDI; FLORIN, LUIZ GUILHERME; ERVOLINO, EDILSON. Influence of adjuvant therapy with green tea extract in the treatment of experimental periodontitis. ARCHIVES OF ORAL BIOLOGY, v. 102, p. 65-73, JUN 2019. (14/11427-8, 14/20163-4)

NOVAES, VIVIAN CRISTINA NORONHA; ERVOLINO, EDILSON; FERNANDES, GIOVANI LOPES; CUNHA, CLARA POSSARLE; THEODORO, LETICIA HELENA; GARCIA, VALDIR GOUVEIA; DE ALMEIDA, JULIANO MILANEZI. Influence of the treatment with the antineoplastic agents 5-fluorouracil and cisplatin on the severity of experimental periodontitis in rats. SUPPORTIVE CARE IN CANCER, v. 30, n. 3, OCT 2021. (14/11427-8)

FIORIN, LUIZ GUILHERME; MATHEUS, HENRIQUE RINALDI; ERVOLINO, EDILSON; CANCIANI, ELENA; PELLEGRINI, GAIA; DELLAVIA, CLAUDIA; MAIORANA, CARLO; DE ALMEIDA, JULIANO MILANEZI. Tamoxifen improves homeostasis in the peri-implant bone remodeling of osseointegrated titanium implants. JOURNAL OF PERIODONTAL RESEARCH, v. 57, n. 4, p. 11-pg., 2022-08-01. (19/24825-5, 14/11427-8, 17/11688-4)