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Interaction between light, hormonal and nitric oxide signaling during plastidial biogenesis and differentiation and nutraceutical compounds accumulation in tomato fruits

Grant number: 13/18056-2
Support type:Regular Research Grants
Duration: October 01, 2014 - March 31, 2017
Field of knowledge:Biological Sciences - Botany
Principal Investigator:Luciano Freschi
Grantee:Luciano Freschi
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Increases in food nutritional quality has became an important goal of modern programs of genetic improvement of plants and, in this context, tomato is increasingly being adopted as a model species for studies focused on the biosynthesis and accumulation of nutritional/nutraceutical compounds in fresh fruits. Relatively recent evidences have indicated that the levels of carotenoids, flavonoids, phenylflavonoids and vitamins can be simultaneously improved in tomato by changes in light and hormonal perception and transduction, resulting in modifications in both biosynthesis and storage capacity of these compounds, which, in many cases, are associated with alterations in the abundance, size and ultrastructure of plastids (chloroplasts and chromoplasts). Besides light and phytohormones, recent studies have also suggested a possible role for the free radical nitric oxide (NO) in the regulation of these physiological responses. Considering the lack of knowledge on the mechanistic interactions between light, hormones and NO signaling during the control of these responses, the present project aims to analyze, in a integrative way, the influence of four hormonal classes (auxins, cytokinins, abscisic acid and ethylene), NO and light signaling on plastidial biogenesis and differentiation, as well as the accumulation of nutraceutical compounds in tomato fruits. Firstly, the temporal and spatial patterns of hormonal and NO signaling during chloroplast biogenesis and development in immature fruits of tomato photomorphogenic mutants will be characterized. Then, the influence of the hormonal and NO signaling on the chloroplast-to-chromoplast conversion in fruits of photomorphogenic mutants exposed to different light treatments will also be investigated. Finally, the effects of silencing phytochrome A, B1 and B2 genes, specifically in the fruit tissues, on the plastidial development and on the accumulation of nutraceutical compounds will also be analyzed. In all cases, the impacts of light signaling on the hormonal profile and on the NO metabolism will be analyzed in parallel to the characterization of the light influence on the plastidial abundance and ultrastructure, metabolic profile (organic acids, sugars, aminoacids), nutraceutical compound profile (carotenoids, flavonoids, vitamins C and E), as well as in terms of transcriptional profile of key enzymes in the biosynthesis of these high nutritional quality compounds. Taken together, the approaches selected for this project not only have the potential to increase the current knowledge about the involvement of light, phytohormones and NO on the control of plastidial biogenesis and nutritional/nutraceutical quality of tomato fruits, but also have the potential to increase the understanding about the complex crosstalk between plant hormones, NO and the signal transduction pathways triggered by light stimuli. In addition, these studies can also help to direct future plant breeding programs targeted to improve the levels of health-promoting substances in tomato and other fresh fruits. (AU)

Scientific publications (11)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZSOGON, AGUSTIN; CERMAK, TOMAS; NAVES, EMMANUEL REZENDE; NOTINI, MARCELA MORATO; EDEL, KAI H.; WEINL, STEFAN; FRESCHI, LUCIANO; VOYTAS, DANIEL F.; KUDLA, JORG; PEREIRA PERES, LAZAR EUSTIQUIO. De novo domestication of wild tomato using genome editing. NATURE BIOTECHNOLOGY, v. 36, n. 12, p. 1211+, DEC 2018. Web of Science Citations: 46.
CRUZ, ALINE BERTINATTO; BIANCHETTI, RICARDO ERNESTO; RODRIGUES ALVES, FREDERICO ROCHA; PURGATTO, EDUARDO; PEREIRA PERES, LAZARO EUSTAQUIO; ROSSI, MAGDALENA; FRESCHI, LUCIANO. Light, Ethylene and Auxin Signaling Interaction Regulates Carotenoid Biosynthesis During Tomato Fruit Ripening. FRONTIERS IN PLANT SCIENCE, v. 9, SEP 18 2018. Web of Science Citations: 9.
BIANCHETTI, RICARDO ERNESTO; LIRA, BRUNO SILVESTRE; MONTEIRO, SCARLET SANTOS; DEMARCO, DIEGO; PURGATTO, EDUARDO; ROTHAN, CHRISTOPHE; ROSSI, MAGDALENA; FRESCHI, LUCIANO. Fruit-localized phytochromes regulate plastid biogenesis, starch synthesis, and carotenoid metabolism in tomato. Journal of Experimental Botany, v. 69, n. 15, p. 3573-3586, JUL 10 2018. Web of Science Citations: 11.
CORPAS, FRANCISCO J.; FRESCHI, LUCIANO; RODRIGUEZ-RUIZ, MARTA; MIOTO, PAULO T.; GONZALEZ-GORDO, SALVADOR; PALMA, JOSE M. Nitro-oxidative metabolism during fruit ripening. Journal of Experimental Botany, v. 69, n. 14, SI, p. 3449-3463, JUN 22 2018. Web of Science Citations: 27.
SILVA, WILLIAN B.; VICENTE, MATEUS H.; ROBLEDO, JESSENIA M.; REARTES, DIEGO S.; FERRARI, RENATA C.; BIANCHETTI, RICARDO; ARAUJO, WAGNER L.; FRESCHI, LUCIANO; PERES, LAZARO E. P.; ZSOGON, AGUSTIN. SELF-PRUNING Acts Synergistically with DIAGEOTROPICA to Guide Auxin Responses and Proper Growth Form. Plant Physiology, v. 176, n. 4, p. 2904-2916, APR 2018. Web of Science Citations: 6.
BIANCHETTI, RICARDO ERNESTO; CRUZ, ALINE BERTINATTO; OLIVEIRA, BRUNA SOARES; DEMARCO, DIEGO; PURGATTO, EDUARDO; PEREIRA PERES, LAZARO EUSTQUIO; ROSSI, MAGDALENA; FRESCHI, LUCIANO. Phytochromobilin deficiency impairs sugar metabolism through the regulation of cytokinin and auxin signaling in tomato fruits. SCIENTIFIC REPORTS, v. 7, AUG 10 2017. Web of Science Citations: 9.
MIOTO, PAULO TAMASO; RODRIGUEZ-RUIZ, MARTA; MOT, AUGUSTIN CATALIN; ZUCCARELLI, RAFAEL; CORPAS, FRANCISCO J.; FRESCHI, LUCIANO; MERCIER, HELENICE. Alternative fluorimetric-based method to detect and compare total S-nitrosothiols in plants. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 68, p. 7-13, AUG 1 2017. Web of Science Citations: 1.
ZUCCARELLI, RAFAEL; COELHO, ALINE C. P.; PERES, LAZARO E. P.; FRESCHI, LUCIANO. Shedding light on NO homeostasis: Light as a key regulator of glutathione and nitric oxide metabolisms during seedling deetiolation. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 68, p. 77-90, AUG 1 2017. Web of Science Citations: 2.
ASGHER, MOHD; PER, TASIR S.; MASOOD, ASIM; FATMA, MEHAR; FRESCHI, LUCIANO; CORPAS, FRANCISCO J.; KHAN, NAFEES A. Nitric oxide signaling and its crosstalk with other plant growth regulators in plant responses to abiotic stress. Environmental Science and Pollution Research, v. 24, n. 3, p. 2273-2285, JAN 2017. Web of Science Citations: 27.
MELO, NIELDA K. G.; BIANCHETTI, RICARDO E.; LIRA, BRUNO S.; OLIVEIRA, PAULO M. R.; ZUCCARELLI, RAFAEL; DIAS, DEVISSON L. O.; DEMARCO, DIEGO; PERES, LAZARO E. P.; ROSSI, MAGDALENA; FRESCHI, LUCIANO. Nitric Oxide, Ethylene, and Auxin Cross Talk Mediates Greening and Plastid Development in Deetiolating Tomato Seedlings. Plant Physiology, v. 170, n. 4, p. 2278-2294, APR 2016. Web of Science Citations: 19.
RODRIGUES, MARIA A.; BIANCHETTI, RICARDO E.; FRESCHI, LUCIANO. Shedding light on ethylene metabolism in higher plants. FRONTIERS IN PLANT SCIENCE, v. 5, DEC 1 2014. Web of Science Citations: 16.

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