Gene expression, cell differentiation and gastric functions: can alimentary changes during postnatal development disturb the growth program in rats?

• Grant number: 14/21449-9
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2015
• End date: July 31, 2017
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Patrícia Gama
• Grantee: Patrícia Gama
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Daniela Ogias

Abstract

The development of the gastrointestinal tract depends on proper suckling-weaning transition. In the gastric mucosa, hormones and milk-born peptides regulate cell proliferation, differentiation and death. Our hypothesis is that suckling is one of the main regulatory agents during development, and so, changes in dietary pattern or conditions can alter the expression of genes that regulate stem cell niche, cell proliferation and differentiation and consequently the growth, maturation and function of gastric mucosa in adult rats. In order to test this idea, this proposal aims to evaluate how dietary patterns influence the expression of genes that control growth, development and function of epithelial cells in the adulthood. During postnatal development, Wistar rats will be submitted to either maternal separation or early weaning and the stomach will be collected at 2nd, 3rd, 5th and 10th weeks to: extract RNA from gastric corpus region, which will be lately analyzed by qPCR; extract proteins for western and immunoblotting, enzyme activity and morphological evaluation under light and electron microscopy. Results will be put together according to experiments and statistically analyzed. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OGIAS, DANIELA; RATTES, ISADORA C.; HOSOYA, LARISSA Y. M.; ZULIAN, JULIANA G.; YAN, CHAO YUN IRENE; GAMA, PATRICIA. Neonatal- maternal separation primes zymogenic cells in the rat gastric mucosa through glucocorticoid receptor activity. SCIENTIFIC REPORTS, v. 8, JUN 29 2018. (12/10948-9, 11/17415-3, 14/21449-9, 10/03076-0, 13/18459-0)

OGIAS, D.; RATTES, I.; ZULIAN, J. G.; RIGONATTI, C. A.; GAMA, P.. Can gastric cell differentiation be reprogrammed by early life stress?. MOLECULAR BIOLOGY OF THE CELL, v. 26, p. 2-pg., 2015-01-01. (14/21449-9, 11/17415-3, 12/10948-9)

TELES SILVA, MELISSA; MESQUITA DA SILVA, KETHLEEN; CAMPOS RATTES, ISADORA; MARIA AGOSTINI ZONTA, GIZELA; VASQUES DA COSTA, ALINE; GALVAO FIGUEREDO COSTA, RAQUEL; KAREN CORDEIRO NOGUEIRA, LUDIMILA; OGIAS, DANIELA; GAMA, PATRICIA. Immediate and Late Effects of Early Weaning on Rat Gastric Cell Differentiation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 1, JAN 1 2020. (11/17415-3, 12/10948-9, 14/21449-9, 16/21944-5, 18/24972-5, 18/07782-8)

ZULIAN, JULIANA GUIMARAES; MASSARENTI HOSOYA, LARISSA YUKARI; FIGUEIREDO, PRISCILA MOREIRA; OGIAS, DANIELA; OSAKI, LUCIANA HARUMI; GAMA, PATRICIA. Corticosterone activity during early weaning reprograms molecular markers in rat gastric secretory cells. SCIENTIFIC REPORTS, v. 7, MAR 31 2017. (13/18459-0, 11/17415-3, 14/21449-9, 10/03076-0)

MESQUITA DA SILVA, KETHLEEN; RATTES, ISADORA CAMPOS; PEREIRA, GIZELA MARIA AGOSTINI; GAMA, PATRICIA. Lifelong Adaptation of Gastric Cell Proliferation and Mucosa Structure to Early Weaning-Induced Effects. FRONTIERS IN PHYSIOLOGY, v. 12, SEP 13 2021. (14/21449-9, 18/05064-0, 18/07782-8)

GAMA, PATRICIA; SILVA, MELISSA TELES; SILVA, KETHLEEN MESQUITA; AGOSTINI ZONTA, GIZELA M.; ZULIAN, JULIANA GUIMARAES; OGIAS, DANIELA. Gene expression, cell differentiation and gastric functions: can dietary changes during postnatal development disturb the growth program in rats?. FASEB JOURNAL, v. 31, p. 1-pg., 2017-04-01. (14/21449-9)