In vitro study of the behavior of fibroblasts derived from venous ulcers in the presence of oxidized regenerated cellulose and collagen

• Grant number: 14/21443-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2015
• End date: July 31, 2018
• Field of knowledge: Health Sciences - Nursing - Medical-Surgical Nursing
• Principal Investigator: Cesar Isaac
• Grantee: Cesar Isaac
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Júlia Teixeira Nicolosi ; Suzana Beatriz Verissimo de Mello ; Viviane Fernandes de Carvalho

Abstract

Chronic ulcers of venous origin affect 1 % of the world population and corresponds to 75 % of all chronic ulcers. The chronicity of venous ulcer cause pain to the patient and away from the dysfunction of their daily activities . Biochemical factors of chronic venous ulcer are not yet fully understood but it is known that this ulcer presents imbalance between the production of matrix metalloproteinases ( MMP ) and tissue factors inhibiting metalloproteinases ( TIMPs ) , abnormal metabolism of collagen , senescent fibroblasts and changes in the action of growth such as transforming growth factor ² ( TGF - ² ) factors . Given the flaws in the tissue repair process , several therapies have been developed especially for dressings that interact with the wound environment . Among these dressings literature has emphasized the oxidized regenerated cellulose ( oxidized regenerated cellulose - ORC ) / collagen whose function is to change the amount of MMPs / TIMPs present in the wound bed This study will evaluate the behavior in vitro of human fibroblasts derived from bed of venous ulcers in the presence of ORC / collagen as the morphology , viability , synthesis of type I collagen , type III collagen , TGF - ² , MMP - 1 , MMP - 3 , TIMP - 1 and TIMP - 3 . These cells will be in half with solution ORC / collagen and evaluated by , MTS assay , curve fibroblast proliferation and immunofluorescence electron microscopy . (AU)