Molecular studies of deubiquitinating enzymes and its involvement in TGFb signaling pathway in the development of the reproductive system of Schistosoma mansoni

• Grant number: 16/06769-2
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Vanderlei Rodrigues
• Grantee: Vanderlei Rodrigues
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Lizandra Guidi Magalhães ; Renata Guerra de Sá Cota

Abstract

This research project seeks to analyze the effects of some substances in the oviposition and the involvement of the same in the signaling pathways of oogenesis the parasite Schistosoma mansoni. For this, the reproductive system and thus egg production in vitro by parasite couples will be analyzed in the presence of deubiquitinating enzymes inhibitors (DUBs) (b-AP15 and PR-619), CNT-siRNA for siRNA Dubs, PSI (proteasome inhibitor), PAP-1 (proteasome activator), as well as effects on the morphology of the component parts of the reproductive organs of males and females parasites, apoptotic activity and expression of TGFb signaling molecules transcripts involved in reproductive growth of this parasite . Thus, the research project will allow its development to obtain advances in understanding this signaling pathway involved in the development and maturation of the reproductive system of the parasite. This process of oogenesis and oviposition are important biological factors for the development of schistosomal pathology in the human host, identification of these molecules can be considered as a future therapeutic targets of this disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO PATROCINIO, ANDRESSA BARBAN; RODRIGUES, VANDERLEI; MAGALHAES, LIZANDRA GUIDI. P53: Stability from the Ubiquitin-Proteasome System and Specific 26S Proteasome Inhibitors. ACS OMEGA, v. 7, n. 5, p. 8-pg., 2022-01-27. (16/06769-2)

DO PATROCINIO, ANDRESSA BARBAN; CABRAL, FERNANDA JANKU; BRANDAO BITENCOURT, ANDRE LUIZ; BRIGATO, OLINDA MARA; MAGALHAES, LIZANDRA GUIDI; DE LIMA PAULA, LUCAS ANTONIO; FRANCO, LARISSA; GUERRA-SA, RENATA; RODRIGUES, VANDERLEI. Inhibition of 19S proteasome deubiquitinating activity in Schistosoma mansoni affects viability, oviposition, and structural changes. Parasitology Research, v. 119, n. 7, MAY 2020. (16/06769-2, 17/07364-9)

DO PATROCINIO, ANDRESSA BARBAN; CABRAL, FERNANDA JANKU; DE PAIVA, THALES HENRIQUE; MAGALHAES, LIZANDRA GUIDI; DE LIMA PAULA, LUCAS ANTONIO; BRIGATO, OLINDA MARA; GUERRA-SA, RENATA; RODRIGUES, VANDERLEI. Deubiquitinating enzymes as possible drug targets for schistosomiasis. Acta Tropica, v. 217, MAY 2021. (16/06769-2, 17/07364-9)