EVALUATION OF SOROLOGIC MARKERS, GENETIC AND EPIGENETIC ABNORMALITIES AS PROGNOSIS INDICATORS OF TUMORS IN DOGS

• Grant number: 16/00128-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2016
• End date: March 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Mirela Tinucci Costa
• Grantee: Mirela Tinucci Costa
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Andrigo Barboza de Nardi ; Áureo Evangelista Santana ; Felipe Augusto Ruiz Sueiro ; Flávio Vieira Meirelles ; Renee Laufer Amorim ; sabryna gouveia calazans

Abstract

The incidence of tumors in dogs has been growing each Day, since their life expectancy is growing too. Mammary tumors, cutaneous and multicentric lymphoma has an elevated prevalence in our clinical routine. Thus, this study aim to evaluate prognostic factors enrolled in the pathogenesis of this neoplasms. For mammary tumors, 30 animals will be used, in which will be performed the mensuration of CA 15-3, CA 125, CEA and alphafetoprotein serologic markers, as well as, evaluation of acetylation of H3 and H4 histones and other enzymes responsible for acetylation in tissues. It will be selected 20 dogs with cutaneous lymphoma to perform the mensuration of IL2R and CA 125 sorologic markers and the evaluation of epigenetic alterations in skin samples. To multicentric large-B-cell lymphoma (DLBCL) will be selected 15 dogs by the evaluation of transcriptome profile obtained by RNASeq technique. According to results obtained, statistical analysis will be applied to find a correlation among patients prognosis and the concentration of serologic markers, genetic and epigenetic alterations, expressed by disease free interval, relapse and survival time. With this study we aim to standardize sorologic markers that can be reliable to diagnosis and follow up to predict remission or relapse in patients with mammary tumors or cutaneous lymphoma. We also aim to research epigenetic alterations in histones and enzymes responsible for its acetylation and its impact in survival time, as well as, evaluate genes expressed in DLBCL as prognostic indicators. We believe that with this study we Will be able to monitor patients and predict the tumoral behavior, remission and relapse, survival time and tumor aggressiveness, issues that are few explored in veterinary oncology. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

IGOR LUIZ SALARDANI SENHORELLO; MIRELA TINUCCI-COSTA. Modificações epigenéticas de histonas e sua relação com o câncer: uma visão da medicina comparada. Ciência Rural, v. 53, n. 11, 2023-05-15. (16/00128-5)

I.C. CANAVARI; I.L.S. SENHORELLO; C. GOLONI; F.A.R. SUEIRO; M. TINUCCI-COSTA. [Níveis séricos do receptor solúvel de interleucina-2 (sIL-2R) e antígeno do câncer 125 (CA 125) em cães diagnosticados com linfoma cutâneo]. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 73, n. 3, p. 747-751, 2021-06-21. (16/00128-5)

SENHORELLO, IGOR L. S.; TERRA, ERIKA M.; SUEIRO, FELIPE A. R.; FIRMO, BRUNA F.; ANAI, LETICIA A.; GOLONI, CAMILA; CANAVARI, ISABELA C.; AMPUERO, ROBERTO A. N.; PEREIRA, RODRIGO S.; TINUCCI-COSTA, MIRELA. Clinical value of carcinoembryonic antigen in mammary neoplasms of bitches. VETERINARY AND COMPARATIVE ONCOLOGY, v. 18, n. 3, NOV 2019. (15/15015-9, 16/00128-5)