Development of functional thin films via layer-by-layer technique aiming selective cell capture for therapeutic and diagnostic purposes

• Grant number: 16/19976-6
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2016
• End date: February 28, 2019
• Field of knowledge: Engineering - Chemical Engineering - Chemical Technology
• Principal Investigator: Marisa Masumi Beppu
• Grantee: Marisa Masumi Beppu
• Host Institution: Faculdade de Engenharia Química (FEQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Cancer is the second leading cause of death, only behind heart disease. In response to some cancers, lymphocytes can migrate to the tumor site for therapeutic effect. Previous work has demonstrated that these immune cells can be used as drug carriers through the attachment of 7-10 µm diameter polymer patches. These cellular backpacks have the ability to encapsulate and to release drugs while successfully avoiding phagocytic internalization, remaining on the cell surface with minimal reduction in cell viability. Their fabrication involves layer-by-layer (LbL) polymer deposition onto a photopatterned array to create a stacked composite of three stratified multilayer systems: a sacrifice region for easy detachment from substrate, a drug payload region, and a cell adhesive region. Previous work employed chitosan/hyaluronicacid, (CHI/HA), multilayers as the cell adhesive region due to the high affinity of hyaluronate for the CD44 receptors at the cells' surface. In this work we propose to investigate the potential selectivity of the (CHI/HA) films by studying their interactions with circulating immune system cells in clinically relevant conditions, such as whole blood, since previous works employed isolated lymphocyte cells in buffer solutions. Another approach is the isolation of tumor cells. Of note, preliminary tests demonstrated that three different prostate cancer cell lines presented better adhesion than healthy prostate cells on films topped with HA (p < 0.05) over uncoated glass slides. This approach may be used as a mechanism for the capture and cell isolation for drug delivery and diagnosis purposes. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROCHA NETO, J. B. M.; GOMES NETO, R. J.; BATAGLIOLI, R. A.; TAKETA, T. B.; PIMENTEL, S. B.; BARATTI, M. O.; COSTA, C. A. R.; CARVALHO, H. F.; BEPPU, M. M.. Engineering the surface of prostate tumor cells and hyaluronan/chitosan multilayer films to modulate cell-substrate adhesion properties. International Journal of Biological Macromolecules, v. 158, p. 197-207, SEP 1 2020. (16/19976-6, 16/10193-9, 13/05135-1)

BATAGLIOLI, R. A.; TAKETA, T. B.; ROCHA NETO, J. B. M.; LOPES, L. M.; COSTA, C. A. R.; BEPPU, M. M.. Analysis of pH and salt concentration on structural and model-drug delivery properties of polysaccharide-based multilayered films. Thin Solid Films, v. 685, p. 312-320, SEP 1 2019. (13/05135-1, 16/19976-6, 16/10193-9)

ROCHA NETO, J. B. M.; TAKETA, T. B.; BATAGLIOLI, R. A.; PIMENTEL, S. B.; SANTOS, D. M.; FIAMINGO, A.; COSTA, C. A. R.; CAMPANA-FILHO, S. P.; CARVALHO, H. F.; BEPPU, M. M.. Tailored chitosan/hyaluronan coatings for tumor cell adhesion: Effects of topography, charge density and surface composition. Applied Surface Science, v. 486, p. 508-518, AUG 30 2019. (16/19976-6, 13/05135-1, 16/10193-9)