Evaluation of immunoregulatory microenvironment of oral premalignant lesions, squamous cell carcinomas and oropharyngeal carcinomas related to HPV or not.

• Grant number: 15/07727-9
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2017
• End date: February 28, 2019
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Paulo Henrique Braz da Silva
• Grantee: Paulo Henrique Braz da Silva
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Bengt Olof Valter Hasséus ; Juliana Lucena Schussel ; Suzana Cantanhede Orsini Machado de Sousa

Abstract

The study of tumor microenvironment is extremely important for understanding the individual immune response to neoplastic cells. Immunotolerance is a physiological mechanism by which the immune system self-regulate, by inhibiting the effector cell response, making it difficult for immune response initiation. This mechanism inhibits the development of autoimmune diseases, controlling and modulating chronic inflammation. Immature dendritic cells, regulatory T cells and numerous immunosuppressive cytokines and chemokines such as IL-10 and MIP3 alpha play a central role in this context. However, it is known that some tumors, especially those which have a viral etiological factor involved are capable of inducing an immunotolerant environment, thus inhibiting the immune response effectively preventing the recognition and destruction of neoplastic cells. Some studies have reported the existence of tolerant microenvironment in neoplastic lesions of the cervix associated with HPV. Given the above, the aim is to analyze the microenvironment of potentially malignant lesions and oropharyngeal carcinomas related to HPV or not, for the presence of immature dendritic cells, FOXP3 + regulatory T cells and expression of MIP3 alpha. (AU)

Scientific publications

(25)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MEDINA, J. B.; ANDRADE, N. S.; DE PAULA EDUARDO, F.; BEZINELLI, L.; FRANCO, J. B.; GALLOTTINI, M.; BRAZ-SILVA, P. H.; ORTEGA, K. L.. Bleeding during and after dental extractions in patients with liver cirrhosis. International Journal of Oral and Maxillofacial Surgery, v. 47, n. 12, p. 1543-1549, DEC 2018. (15/07727-9)

DE SANTANA SARMENTO, DMITRY JOSE; PALMIERI, MICHELLE; GALVAO, GUSTAVO SOUZA; TOZETTO-MENDOZA, TANIA REGINA; DO CANTO, CYNTHIA MOTTA; PIERROTTI, LIGIA CAMERA; DAVID-NETO, ELIAS; AGENA, FABIANA; GALLOTTINI, MARINA; PANNUTI, CLAUDIO SERGIO; et al. BK virus salivary shedding and viremia in renal transplant recipients. Journal of Applied Oral Science, v. 27, 2019. (15/07727-9)

PALMIERI, MICHELLE; ORNAGHI, MARIANA; DE OLIVEIRA MARTINS, VICTOR ADRIANO; CORREA, LUCIANA; BRANDAO, THAIS BIANCA; DO PRADO RIBEIRO, ANA CAROLINA; SUMITA, LAURA MASAMI; TOZETTO-MENDOZA, TANIA REGINA; PANNUTI, CLAUDIO SERGIO; BRAZ-SILVA, PAULO HENRIQUE. Oral shedding at human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia. JOURNAL OF ORAL MICROBIOLOGY, v. 10, MAY 28 2018. (15/07727-9, 15/03906-6, 12/04303-5)

DE OLIVEIRA, MARCIO AUGUSTO; REIS, BRUNA; PALLOS, DEBORA; KIM, YEON JUNG; BRAZ-SILVA, PAULO HENRIQUE; MARTINS, FABIANA. The Importance of Histopathological Examination to the Final Diagnosis of Peripheral Odontogenic Tumors: A Case Report of a Peripheral Odontoma. CASE REPORTS IN DENTISTRY, v. 2019, SEP 8 2019. (15/07727-9, 18/02568-8)

DE SANTANA SARMENTO, DMITRY JOSE; TOZETTO-MENDOZA, TANIA REGINA; FERNANDES DE SOUZA, ANA CAROLINA MAMANA; MACIEL, RAFAEL; PAIAO, HEUDER; LIMA, SILVIA HELENA; CRISTELLI, MARINA; MEDINA DE ABREU PESTANA, JOSE OSMAR; BRAZ-SILVA, PAULO HENRIQUE; GALLOTTINI, MARINA. Herpesviruses oral shedding and viremia in renal transplant recipients: A longitudinal study. TRANSPLANT INFECTIOUS DISEASE, v. 22, n. 4, JUN 2020. (17/07778-8, 15/07727-9)

BERGAMINI, MARIANA LOBO; MARDEGAN, ANDRESSA PINTO; DE ROSA, CATHARINA SIMIONI; PALMIERI, MICHELLE; DE SANTANA SARMENTO, DMITRY JOSE; NAKAMURA HIRAKI, KAREN RENATA; FERREIRA COSTA, ANDRE LUIZ; HASSEUS, BENGT; JONASSON, PETER; BRAZ-SILVA, PAULO HENRIQUE. Presence of langerhans cells, regulatory T cells (Treg) and mast cells in asymptomatic apical periodontitis. BRAZILIAN ORAL RESEARCH, v. 34, p. 10-pg., 2020-01-01. (15/07727-9)

DA SILVA MARCELINO, BARBARA M. R.; PARISE, GUILHERME K.; DO CANTO, ALAN M.; SASSI, LAURINDO M.; SARMENTO, DMITRY J. S.; COSTA, ANDRE L. F.; HASSEUS, BENGT; KJELLER, GORAN; SCHUSSEL, JULIANA L.; BRAZ-SILVA, PAULO H.. Comparison of Immunohistochemistry and DNA Sequencing for BRAF V600E Mutation Detection in Mandibular Ameloblastomas. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, v. 29, n. 5, p. 4-pg., 2021-05-01. (17/18576-7, 15/07727-9)

GANESH, DIVYA; SREENIVASAN, PRATHIMA; OHMAN, JENNY; WALLSTROM, MATS; BRAZ-SILVA, PAULO HENRIQUE; GIGLIO, DANIEL; KJELLER, GORAN; HASSEUS, BENGT. Potentially Malignant Oral Disorders and Cancer Transformation. ANTICANCER RESEARCH, v. 38, n. 6, p. 3223-3229, JUN 2018. (15/07727-9)

FEIJO ROSSETO, JOSE HENRIQUE; TENORIO, JEFFERSON ROCHA; MAMANA, ANA CAROLINA; TOZETTO-MENDOZA, TANIA REGINA; ANDRADE, NATALIA SILVA; BRAZ-SILVA, PAULO HENRIQUE; ORTEGA, KAREM L.. Epstein-Barr virus oral shedding and viremia and their association with oral hairy leukoplakia in HIV plus individuals. ORAL DISEASES, AUG 2021. (15/07727-9)

BRAZ-SILVA, PAULO HENRIQUE; DO CANTO, ALAN MOTTA; OLIVEIRA, LETICIA; MARTINS, FABIANA; PEREIRA DA COSTA, ALESSANDRO ANTONIO; COSTA HANEMANN, JOAO ADOLFO. Sclerosing Polycystic Adenosis of Tongue. BULLETIN OF TOKYO DENTAL COLLEGE, v. 59, n. 2, p. 121-125, 2018. (15/07727-9)

BRAZ-SILVA, PAULO HENRIQUE; BERGAMINI, MARIANA LOBO; MARDEGAN, ANDRESSA PINTO; DE ROSA, CATHARINA SIMIONI; HASSEUS, BENGT; JONASSON, PETER. Inflammatory profile of chronic apical periodontitis: a literature review. ACTA ODONTOLOGICA SCANDINAVICA, v. 77, n. 3, p. 173-180, APR 3 2019. (15/07727-9)

MIRANDA-SILVA, WANESSA; DE MOLLA, VINICIUS CAMPOS; KNEBEL, FRANCIELE HINTERHOLZ; TOZETTO-MENDOZA, TANIA REGINA; ARRAIS-RODRIGUES, CELSO; CAMARGO, ANAMARIA ARANHA; BRAZ-SILVA, PAULO HENRIQUE; FREGNANI, EDUARDO RODRIGUES. Oral shedding of herpesviruses and clinical outcomes in hematopoietic stem cell transplant patients. ORAL DISEASES, SEP 2021. (15/07727-9)

MARIANA LOBO BERGAMINI; ANDRESSA PINTO MARDEGAN; CATHARINA SIMIONI DE ROSA; MICHELLE PALMIERI; DMITRY JOSÉ DE SANTANA SARMENTO; KAREN RENATA NAKAMURA HIRAKI; ANDRÉ LUIZ FERREIRA COSTA; BENGT HASSÉUS; PETER JONASSON; PAULO HENRIQUE BRAZ-SILVA. Presence of langerhans cells, regulatory T cells (Treg) and mast cells in asymptomatic apical periodontitis. Brazilian Oral Research, v. 34, 2020-08-28. (15/07727-9)

MIRANDA-SILVA, WANESSA; KNEBEL, FRANCIELE HINTERHOLZ; TOZETTO-MENDOZO, TANIA REGINA; PALMIERI, MICHELLE; DA FONSECA, FELIPE PAIVA; CAMARGO, ANAMARIA ARANHA; BRAZ-SILVA, PAULO HENRIQUE; FREGNANI, EDUARDO RODRIGUES. Herpesviruses in the oral cavity of patients subjected to allogeneic hematopoietic stem cell transplantation and its relationship with oral mucositis. CLINICAL ORAL INVESTIGATIONS, FEB 2020. (15/07727-9)

DA SILVA MARCELINO, BARBARA M. R.; PARISE, GUILHERME K.; DO CANTO, ALAN M.; SASSI, LAURINDO M.; SARMENTO, DMITRY J. S.; COSTA, ANDRE L. F.; HASSEUS, BENGT; KJELLER, GORAN; SCHUSSEL, JULIANA L.; BRAZ-SILVA, PAULO H.. Comparison of Immunohistochemistry and DNA Sequencing for BRAF V600E Mutation Detection in Mandibular Ameloblastomas. APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, v. 29, n. 5, p. 390-393, MAY-JUN 2021. (17/18576-7, 15/07727-9)

COSTA, ANDRE L. F.; SANTOS, BRUNA A.; TORREGROSSA, VINICIUS R.; MIRANDA, ELIANA C. M.; VIGORITO, AFONSO C.; PALMIERI, MICHELLE; RICARDO, ANA L. F.; SARMENTO, DMITRY J. S.; MAMANA, ANA C.; TOZETTO-MENDOZA, TANIA R.; et al. Oral shedding of CMV and HSV-1 in hematopoietic stem cell transplantation patients. ORAL DISEASES, NOV 2020. (15/07727-9)

SUNDBERG, JONAS; OHMAN, JENNY; KORYTOWSKA, MAGDALENA; WALLSTROM, MATS; KJELLER, GORAN; ANDERSSON, MARIA; HORAL, PETER; LINDH, MAGNUS; GIGLIO, DANIEL; KOVACS, ANIKO; et al. High-risk human papillomavirus in patients with oral leukoplakia and oral squamous cell carcinoma-A multi-centre study in Sweden, Brazil and Romania. ORAL DISEASES, v. 27, n. 2, JUL 2020. (15/07727-9, 17/02889-6)

MAMANA, ANA CAROLINA; STINCARELLI, MARIA; SARMENTO, DMITRY JOSE DE SANTANA; MENDES BATISTA, ALEXANDRE; TOZETTO-MENDOZA, TANIA REGINA; GALLOTTINI, MARINA; DE ABREU PESTANA, JOSE OSMAR MEDINA; BRAZ-SILVA, PAULO HENRIQUE; GIANNECCHINI, SIMONE. Detection of polyomavirus microRNA-5p expression in saliva shortly after kidney transplantation. JOURNAL OF ORAL MICROBIOLOGY, v. 13, n. 1, JAN 1 2021. (15/07727-9)

ORTEGA, K. L.; RECH, B. O.; EL HAJE, G. L. C.; GALLO, C. B.; PEREZ-SAYANS, M.; BRAZ-SILVA, P. H.. Do hydrogen peroxide mouthwashes have a virucidal effect? A systematic review. Journal of Hospital Infection, v. 106, n. 4, p. 657-662, DEC 2020. (15/07727-9)

DO CANTO, ALAN MOTTA; REIS DA SILVA MARCELINO, BARBARA MICHAELA; SCHUSSEL, JULIANA LUCENA; WASTNER, BRUNA F.; SASSI, LAURINDO MOACIR; CORREA, LUCIANA; DE FREITAS, RONALDO RODRIGUES; HASSEUS, BENGT; KJELLER, GORAN; LEMOS JUNIOR, CELSO AUGUSTO; et al. Immunohistochemical analysis of BRAF V600E mutation in ameloblastomas. CLINICAL ORAL INVESTIGATIONS, v. 23, n. 2, p. 779-784, FEB 2019. (15/07727-9)

PALLOS, DEBORA; SOUSA, VANESSA; FERES, MAGDA; RETAMAL-VALDES, BELEN; CHEN, TSUTE; CURTIS, MIKE; BOAVENTURA, RICHARDSON MONDEGO; TANAKA, MARCIA HIROMI; SALOMAO, GUSTAVO VARGAS DA SILVA; ZANELLA, LOUISE; et al. alivary Microbial Dysbiosis Is Associated With Peri-Implantitis: A Case-Control Study in a Brazilian Populatio. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JAN 5 2022. (13/08242-3, 15/07727-9)

COSTA, ANDRE L. F.; SANTOS, BRUNA A.; TORREGROSSA, VINICIUS R.; MIRANDA, ELIANA C. M.; VIGORITO, AFONSO C.; PALMIERI, MICHELLE; RICARDO, ANA L. F.; SARMENTO, DMITRY J. S.; MAMANA, ANA C.; TOZETTO-MENDOZA, TANIA R.; et al. Oral shedding of CMV and HSV-1 in hematopoietic stem cell transplantation patients. ORAL DISEASES, v. 27, n. 6, p. 1572-1579, SEP 2021. (15/07727-9)

DANIELA ASSIS DO VALE; LAIS MAGDA ARAÚJO FERRACINI; MARCUS VINÍCIUS DA SILVA RODRIGUES BUENO; ANA CAROLINA MAMANA FERNANDES SOUZA; PAULO HENRIQUE BRAZ-SILVA; KAREM LÓPEZ ORTEGA. Unusual presentation of a disseminated oral HPV infection after combined antiretroviral therapy initiation. Revista do Instituto de Medicina Tropical de São Paulo, v. 61, 2019-10-10. (15/07727-9)

MIRANDA-SILVA, WANESSA; KNEBEL, FRANCIELE HINTERHOLZ; TOZETTO-MENDOZO, TANIA REGINA; PALMIERI, MICHELLE; DA FONSECA, FELIPE PAIVA; CAMARGO, ANAMARIA ARANHA; BRAZ-SILVA, PAULO HENRIQUE; FREGNANI, EDUARDO RODRIGUES. Herpesviruses in the oral cavity of patients subjected to allogeneic hematopoietic stem cell transplantation and its relationship with oral mucositis. CLINICAL ORAL INVESTIGATIONS, v. 24, n. 10, p. 12-pg., 2020-02-08. (15/07727-9)

BATISTA, ALEXANDRE MENDES; CAETANO, MATHEUS W.; STINCARELLI, MARIA A.; MAMANA, ANA C.; ZERBINATI, RODRIGO MELIM; SARMENTO, DMITRY J. S.; GALLOTTINI, MARINA; CAIXETA, V, RAFAEL A.; MEDINA-PESTANA, JOSE; HASSEUS, BENGT; et al. Quantification of torque teno virus (TTV) DNA in saliva and plasma samples in patients at short time before and after kidney transplantation. JOURNAL OF ORAL MICROBIOLOGY, v. 14, n. 1, JAN 1 2022. (21/03563-2, 17/07778-8, 15/07727-9)