Study of pathogenicity of chicken enteric virus (chicken parvovirus, ANV and chicken astrovirus)in experimentally infected chicken

• Grant number: 17/05296-6
• Support Opportunities: Multi-user Equipment Program
• Start date: June 01, 2017
• End date: May 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Antonio José Piantino Ferreira
• Grantee: Antonio José Piantino Ferreira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/08560-5 - Pathogenicity study of chicken enteric viruses (Astrovirus, avian nephritis virus and Parvovirus) in experimentally infected chickens, AP.R
• EMU web page: http://www.cadifmvzusp.com.br/index.php/ct-menu-item-39
• Type of equipment: Caracterização e Análises de Amostras - Proteínas/Ácidos nuclêicos - PCR
• Manufacturer: ThermoFischer
• Model: qPCR

Abstract

Chicken astrovírus (CAstV), avian nephritis virus (ANV) and chicken parvovirus (CHPV)are virus that were related with enteric problems in chicken commercial flocks, beingidentified in chickens with signs of enteric diseases. Enteric viruses that may be relatedwith enteric problems that affect to poultry and cause important economic losses causedby lack of feed, the disparity in growth and death. These three viruses are not very knownand are investigated as causer of enteric problems, especially of diarrhea, sign relatedwith to the runting and stunting syndrome and lose weight in commercial flocks. InBrazil, these viruses were detected and isolated from hens and chickens both health orwith enteric problems. The involvement of these viruses as cause of enteric diseases is notunderstood, and still not determined if one virus or the combination between those is thecause of enteric diseases. The lack of knowledge of pathogenesis of these virusesbecomes important their molecular characterization and the pathogenicity determinationin vivo, because this information contributes for better knowledge of characteristics ofenteric diseases. Viral strains of chicken astrovírus, avian nephritis virus and chickenparvovirus isolated in chicken embryonated eggs (SPF), in Brazil, at the Laboratory ofAvian Pathology (LABOR) from Veterinary College of the University Sao Paulo(Mettifogo et al., The Scientific World Journal, 2014:450423. Doi 10.1155/2014/450423),from hens or chickens commercial flocks with enteric problems, will be selected formaking the molecular characterization of these viruses. As well as, the pathogenicitydetermination by an experimental infection using the viruses individually and in theircombinations. The present work has as aims: 1) to use of real time RT-PCR and real timePCR by both viral identification and viral quantification; 2) to determinate byexperimental infection if individually the virus or their combinations development or notthe enteric diseases in broiler, 3) to determinate the pathogenicity of these viruses in vivo. (AU)