Spinal cord injury and male infertility: effect of probenecid treatment on seminal toxicity of prostatic origin.

• Grant number: 17/14346-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2018
• End date: January 31, 2020
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Mariana Camargo
• Grantee: Mariana Camargo
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Ricardo Pimenta Bertolla

Abstract

Men with spinal cord injury face many difficulties when trying to have children. Besides erectile and ejaculatory dysfunction, which can be effectively treated, these men also present low semen quality. In order to better understand the mechanisms of which these men present low semen quality, it was verified that the seminal plasma present toxicity caused by inflammasome complex. This complex can be blocked by oral administration of probenecid, increasing progressive motility in these men. After seminal plasma proteomic studies of oral probenecid treated men, realized in our laboratory, it was possible to suggest a panel of proteins that can be responsible for increased sperm motility after treatment. In order to continue to understand the mechanisms affecting low semen quality, the aim of the present study is to investigate the effect of treatment with oral probenecid on seminal toxicity of prostatic origin. For that, 15 spinal cord injury men will be treated with 1000 mg in total by day of oral probenecid. One sample will be collected, by penile vibratory stimulation, before the beginning of treatment and another sample by the end of treatment. One aliquot will be used for semen analysis and the other amount will be used at protein analysis. For protein validation ELISA and Western blotting techniques will be performed. For group comparison paired (Pre x Post) and unpaires (Pre x Control) Student t test will be performed (p < 0.05). With these results we hope to better understand the mechanisms of which the seminal plasma of SCI men is toxic for the sperm. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMARGO, MARIANA; IBRAHIM, EMAD; ABALLA, TEODORO C.; CARDOZO, KARINA H. M.; CARVALHO, VALDEMIR M.; LYNNE, CHARLES M.; BRACKETT, NANCY L.; BERTOLLA, RICARDO P.. Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility. JOURNAL OF SPINAL CORD MEDICINE, FEB 2020. (12/15039-7, 14/17185-6, 17/14346-7)

CAMARGO, MARIANA; IBRAHIM, EMAD; INTASQUI, PAULA; BELARDIN, LARISSA B.; ANTONIASSI, MARIANA P.; LYNNE, CHARLES M.; BRACKETT, NANCY L.; BERTOLLA, RICARDO P.. Seminal inflammasome activity in the adult varicocele. HUMAN FERTILITY, JAN 2021. (17/14346-7)

DE MELO, KARLA PACHECO; CAMARGO, MARIANA. Mechanisms for sperm mitochondrial removal in embryos. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1868, n. 2, FEB 2021. (17/14346-7, 18/25907-2)

CAMARGO, MARIANA; IBRAHIM, EMAD; ABALLA, TEODORO C.; CARDOZO, KARINA H. M.; CARVALHO, VALDEMIR M.; LYNNE, CHARLES M.; BRACKETT, NANCY L.; BERTOLLA, RICARDO P.. Proteomic insight of seminal plasma in spinal cord injured men submitted to oral probenecid treatment for improved motility. JOURNAL OF SPINAL CORD MEDICINE, v. 44, n. 6, p. 966-971, NOV 2 2021. (12/15039-7, 14/17185-6, 17/14346-7)

CAMARGO, MARIANA; IBRAHIM, EMAD; INTASQUI, PAULA; BELARDIN, LARISSA B.; ANTONIASSI, MARIANA P.; LYNNE, CHARLES M.; BRACKETT, NANCY L.; BERTOLLA, RICARDO P.. Seminal inflammasome activity in the adult varicocele. HUMAN FERTILITY, v. 25, n. 3, p. 9-pg., 2021-01-11. (17/14346-7)