| Grant number: | 17/08103-4 |
| Support Opportunities: | Research Projects - Thematic Grants |
| Start date: | February 01, 2018 |
| End date: | January 31, 2024 |
| Field of knowledge: | Biological Sciences - Biochemistry |
| Principal Investigator: | Walter Ribeiro Terra |
| Grantee: | Walter Ribeiro Terra |
| Host Institution: | Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil |
| City of the host institution: | São Paulo |
| Principal investigators | Clelia Ferreira Terra |
| Associated researchers: | Adriana Rios Lopes ; Alberto Augusto Gonçalves de Freitas Castro Ribeiro |
| Associated research grant(s): | 19/21065-0 - Digestion and midgut absorption in 3 insects:an enzymological, transcriptomic , and proteomic approach., AV.BR |
| Associated scholarship(s): | 19/22451-0 - Transporters associated with intestinal physiology in Tenebrio molitor, Rhodnius prolixus and Dysdercus peruvianus, BP.PD |
Abstract
The objective of this project is to describe the spatial organization of the midgut physiological processes and their underlying molecular events of insect types pertaining to different orders. The aim of these studies is to find out general principles from which hypotheses can be advanced in relation to the digestive and absorptive processes and tissue metabolic interactions of insects not previously studied. Such studies are necessary for developing new insect control procedures. The project is divided into 3 parts: (a) Midgut molecular physiology and tissue metabolic interactions, which models will be based on cDNA deep sequencing (obtained by Illumina) associated with data on digestive enzymes, buffering systems, and transporters, etc. The models will be tested by enzyme and transporter inhibitors and by protein silencing with the aid of RNAi; (b) Insect-purified or recombinant digestive enzymes in a search for peculiarities that set them apart from vertebrate enzymes and that led insects to overcome plant defenses; (c) Evolution of insect digestive systems. The present proposal will be improved by studying insect groups not considered before, such as leafhoppers, thrips, and cerambycid beetles, basal and more derived hymenopterans like wasps and ants. Another approach for evolution will be "phylotranscriptomics", the comparative analysis of whole or part of the midgut transcriptomes (obtained by Illumina sequencing) we have available or intend to prepare and phylogenomics of chosen insect genomes. (AU)
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