Evaluation of the primary metabolism of Eucalyptus grandis cambial region and bark

• Grant number: 09/00808-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2009
• End date: June 30, 2012
• Field of knowledge: Agronomical Sciences - Forestry Resources and Forestry Engineering - Forestry
• Principal Investigator: Carlos Alberto Labate
• Grantee: Ilara Gabriela Frasson Budzinski
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

The species of eucalyptus have many favorable characteristics for the production and marketing of its timber, as more adaptation to different climate and soil conditions, high yield and physical characteristics, enabling its widespread use as raw material in industry (pulp and paper, coal vegetable, chemical, pharmaceutical), carpentry (sawmill and wood processing), construction, among others. However, despite its economic importance, little is known about the cellular and molecular processes that involve the formation of wood. Recent studies have reported the identification of genes and proteins metabolism of glycolysis, fermentation and photosynthesis, highly expressed in the cambial region (Celedon et al., 2007 and Carvalho et al., 2008). The results indicate that the glycolytic metabolism may be the major pathway for energy production (ATP) to the cambial region during development and formation of the timber. And also indicate the likely occurrence of fermentative metabolism, mainly due to expression of the enzyme alcohol dehydrogenase. The results are corroborated by the fact that the bark of the trees play a role impermeable to gases such as CO2 and O2, resulting in high concentrations of CO2 exchange in the region, a fact that should cause a significant reduction in mitochondrial respiration. The identification of genes and proteins involved in photosynthetic metabolism indicate the occurrence of the metabolic process in the bark of eucalyptus as described in the literature for arboreal species. Within this context, this project aims to identify the primary metabolites, present in the cambial region, involved in photosynthesis, glycolysis and alcoholic fermentation and inferences about the role of the alcoholic fermentation during the growth of Eucalyptus grandis. From the initial analyzes will be selected genes of interest for transcriptional pattern analysis via Real-time PCR. Thus it will be possible to study the process of wood formation using data from transcriptome, proteome and metabolome analysis. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COTINGUIBA, F.; LOPEZ, S. N.; BUDZINSKI, I. G. F.; LABATE, C. A.; KATO, M. J.; FURLAN, M.. Proteomic profile of Piper tuberculatum (Piperaceae). Brazilian Journal of Biology, v. 78, n. 1, p. 117-124, FEB 2018. (09/00808-2, 06/50086-5, 13/07600-3)

FRASSON BUDZINSKI, ILARA GABRIELA; MOON, DAVID H.; MOROSINI, JULIA SILVA; LINDEN, PERNILLA; BRAGATTO, JULIANO; MORITZ, THOMAZ; LABATE, CARLOS ALBERTO. Integrated analysis of gene expression from carbon metabolism, proteome and metabolome, reveals altered primary metabolism in Eucalyptus grandis bark, in response to seasonal variation. BMC PLANT BIOLOGY, v. 16, JUL 1 2016. (09/00808-2, 08/50361-1)

BUDZINSKI, ILARA G. F.; MOON, DAVID H.; LINDEN, PERNILLA; MORITZ, THOMAS; LABATE, CARLOS A.. Seasonal Variation of Carbon Metabolism in the Cambial Zone of Eucalyptus grandis. FRONTIERS IN PLANT SCIENCE, v. 7, JUN 28 2016. (09/00808-2)

COTINGUIBA, F.; LOPEZ, S. N.; BUDZINSKI, I. G. F.; LABATE, C. A.; KATO, M. J.; FURLAN, M.. Proteomic profile of Piper tuberculatum (Piperaceae). Brazilian Journal of Biology, v. 78, n. 1, p. 8-pg., 2018-02-01. (06/50086-5, 13/07600-3, 09/00808-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BUDZINSKI, Ilara Gabriela Frasson. Evaluation of the primary metabolism of Eucalyptus grandis cambial region and bark. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.