Kinetic studies of cephamycin c purification by ion exchange chromatography and continuous process simulation

• Grant number: 09/03551-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2009
• End date: February 28, 2011
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Principal Investigator: Marlei Barboza Pasotto
• Grantee: Guilherme Youssef Rodriguez
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 05/55079-4 - Production and purification of clavulanic acid, cephamycin C and other bioactive metabolites of Streptomyces, AP.TEM

Abstract

Cephamycin C (CepC) is a beta-lactam compound first isolated from Streptomyces clavuligerus as an inhibitor of the synthesis of the Gram-negative cell wall. Later it was discovered that, besides displaying this inhibitory activity, CepC was resistant to degradation by beta-lactamases. The aim of this work is study of kinetics and equilibrium for Cephamycin C purification by ionic resin. After batch fermentation of S. clavuligerus, the culture broth will be clarified by microfiltration and ultrafiltration, after which the resulting permeate will be subjected to nonspecific adsorption and ion-exchange chromatography on resin. The concentration of CepC was measured by HPLC. In these work the inuence of temperature on the cephamycin C adsorption process will be investigated, involving equilibrium and kinetic studies and carrying out batch shake experiments with the ion exchanger resin Stream Line QXL at four different temperatures and 3 different pH. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YOUSSEF RODRIGUEZ, G.; SACARDO DA SILVA, C.; OSAMU HOKKA, C.; BARBOZA, M.; MENDEZVILAS, A. Extraction of clavulanic acid in aqueous two-phase systems followed by separation through ultrafiltration. MICROORGANISMS IN INDUSTRY AND ENVIRONMENT: FROM SCIENTIFIC AND INDUSTRIAL RESEARCH TO CONSUMER PRODUCTS, v. N/A, p. 5-pg., 2011-01-01. (09/03551-2, 05/55079-4)