Detection of Cryptosporidium parvum in fecal samples of calves using real time PCR

• Grant number: 09/03761-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2009
• End date: June 30, 2011
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcelo Vasconcelos Meireles
• Grantee: Camila Guariz Homem
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Protozoan of the genera Cryptosporidium are coccidia that infect the human beings and other mammals, birds, reptiles and fishes. This protozoan is considered an important ethiologic agent of diarrhea in immunossupessed and in imunocompetents human beings, and in some species of domestic and wild animals. The infection in human beings can be caused by C. hominis, or C. parvum, with many cases described in the literature. In bovines, C. parvum can be responsible for clinical cases of diarrhea and high mortality. The present project has as objective the development of a real time polymerase chain reaction for detection of C. parvum DNA and, after standardization of the protocol, to test the effectiveness of the technique in fecal samples of calves.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HOMEM, CAMILA G.; NAKAMURA, ALEX A.; SILVA, DEUVANIA C.; TEIXEIRA, WESLEN F. P.; COELHO, WILLIAN M. D.; MEIRELES, MARCELO V.. Real-time PCR assay targeting the actin gene for the detection of Cryptosporidium parvum in calf fecal samples. Parasitology Research, v. 110, n. 5, p. 1741-1745, MAY 2012. (09/03761-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

HOMEM, Camila Guariz. Avaliação da PCR em tempo real para detecção de Cryptosporidium parvum em amostras fecais de bezerros: Camila Guariz Homem. -. 2011. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária. Araçatuba Araçatuba.