Mechanical effect on samples of smooth muscle after acute exposure to components found in the pollution

• Grant number: 09/06642-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: September 01, 2009
• End date: August 31, 2011
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Adriano Mesquita Alencar
• Grantee: Mac Gayver da Silva Castro
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

This project aims to study the effect of external agents of normal cell physiology, specifically those found on air pollution, in the mechanics of smooth muscle tissue strips collected from the trachea of male Wistar rats. We will expose separately the samples collected from the trachea smooth muscle of rats to two categories of pollutants. We will washes and centrifugate the product of pollution and separate in two groups: (i) heavy particles and (ii) solute. For mechanical analysis, we will use the equipment of the Air Pollution Laboratory (LIM-HC 05). From the theoretical point of view, we will adapt a model network of springs already established, to meet our experimental conditions. Our hypothesis is that acute exposure of the sample of smooth muscle in these two groups will show opposite effects, ie the heavier particles tend to aggregate with the molecules of integrin and damage the cell skeleton, while the soluble pollution will trigger some form of the contractile apparatus. (AU)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CASTRO, Mac Gayver da Silva. Proposition of a mathematical model to study the mechanical change of the smooth muscle of the trachea of Wistar rats exposed to formaldehyde solution and subjected to increasing doses of a contractile agent. 2012. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.