Action mechanisms and expression analysis of cyclin D1, akt, mTOR and hsp90 proteins in squamous cell carcinoma in vitro after low intensity laser irradiation

• Grant number: 09/08452-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2009
• End date: December 31, 2012
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Suzana Cantanhede Orsini Machado de Sousa
• Grantee: Felipe Fornias Sperandio
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

O presente estudo tem o objetivo de avaliar a expressão e os mecanismos de ação das proteínas akt, ciclina D1, mTOR e hsp90 em cultura de células de carcinoma epidermóide de boca após irradiação com laser em baixa intensidade. Serão utilizados dois comprimentos de onda da luz laser (660 e 780nm), bem como 3 doses distintas (2.05; 3.07 e 6.15 J/cm2). Após irradiação laser, realizar-se-á contagem destas células e análise quantitativa e qualitativa da expressão das proteínas akt, ciclina D1, mTOR e hsp90.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SPERANDIO, FELIPE F.; GIUDICE, FERNANDA S.; CORREA, LUCIANA; PINTO, DECIO S., JR.; HAMBLIN, MICHAEL R.; DE SOUSA, SUZANA C. O. M.. Low-level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway. Journal of Biophotonics, v. 6, n. 10, p. 9-pg., 2013-10-01. (09/08452-2, 09/16183-1)

SPERANDIO, FELIPE F.; GIUDICE, FERNANDA S.; CORREA, LUCIANA; PINTO, JR., DECIO S.; HAMBLIN, MICHAEL R.; DE SOUSA, SUZANA C. O. M.. Low-level laser therapy can produce increased aggressiveness of dysplastic and oral cancer cell lines by modulation of Akt/mTOR signaling pathway. Journal of Biophotonics, v. 6, n. 10, SI, p. 839-847, OCT 2013. (09/16183-1, 09/08452-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SPERANDIO, Felipe Fornias. The expression and action mechanisms of Akt, Hsp90, mTOR and cyclin D1 proteins in cultured cells of squamous cell carcinoma and dysplastic cells after being irradiated with low level laser therapy. 2012. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) São Paulo.