COMPARATIVE INVESTIGATION OF CLINICAL AND MICROBIOLOGICAL DATA BETWEEN GENETICALLY SUSCEPTIBLE INDIVIDUALS AND NOT SUSCETIBLE TO PERIODONTITIS

• Grant number: 09/11371-4
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2010
• End date: February 29, 2012
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Raquel Mantuaneli Scarel Caminaga
• Grantee: Livia Sertori Finoti
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Periodontal disease (PD) is a multifactorial disease caused by the periodontopathogenic microorganisms and influenced by factors such as genetic susceptibility, the reaction of the immune system, smoking and the occurrence of systemic diseases. Recent studies carried out by this group investigated IL8 gene polymorphisms in patients with and without periodontitis. In those studies were observed individuals with a genetic predisposition to periodontitis even after adjusting for covariates such as age, gender, skin color and smoking (results under submission to publication). The purpose of this study is to compare the subgingival microbiota of sites with and without PD, before and 45 days after basic periodontal treatment in genetically susceptible individuals and not susceptible to periodontitis. This way, it will be possible to know if there are qualitative/quantitative differences of periodontopathogenic microorganisms between genetically susceptible individuals and not susceptible to PD. Indeed, if there is differential influence on non-surgical periodontal treatment on the clinical and microbiological parameters between these groups of patients. Eighty individuals will be select and divided into "susceptible" and "not susceptible" to PD. In each group there are patients with and without periodontitis (4 groups, n = 20). The selected patients will be submitted to a new periodontal exams and have their gingival fluid collected before and after 45 days of periodontal treatment. Three periodontopathogenic microorganisms Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola will be analysed by absolute quantification utilizing the Real Time PCR (qPCR). The clinical and microbiological data (at different times) will be submitted to parametric or non-parametric statistical analysis (the significance level of 5%). This study is expected to contribute for better understanding the possible inter-relationship of different factors (clinical, genetic and microbiological) in the PD, once it is a multifactorial disease.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CIRELLI, THAMIRIS; FINOTI, LIVIA S.; CORBI, SAMIA C. T.; ANOVAZZI, GIOVANA; NEPOMUCENO, RAFAEL; ORRICO, SILVANA R. P.; CIRELLI, JONI A.; MAYER, MARCIA P. A.; SCAREL-CAMINAGA, RAQUEL M.. Absolute quantification of Aggregatibacter actinomycetemcomitans in patients carrying haplotypes associated with susceptibility to chronic periodontitis: multifaceted evaluation with periodontitis covariants. PATHOGENS AND DISEASE, v. 75, n. 7, OCT 2017. (09/11371-4, 13/06365-0, 09/11321-7, 09/08773-3)

FINOTI, LIVIA S.; CORBI, SAMIA C. T.; ANOVAZZI, GIOVANA; TEIXEIRA, SILVIA R. L.; CAPELA, MARISA V.; TANAKA, MARCIA H.; KIM, YEON J.; ORRICO, SILVANA R. P.; CIRELLI, JONI A.; MAYER, MARCIA P. A.; et al. Pathogen levels and clinical response to periodontal treatment in patients with Interleukin 8 haplotypes. PATHOGENS AND DISEASE, v. 69, n. 1, p. 21-28, OCT 2013. (09/11371-4, 09/08773-3, 03/10424-0)

FINOTI, L. S.; ANOVAZZI, G.; PIGOSSI, S. C.; CORBI, S. C. T.; TEIXEIRA, S. R. L.; BRAIDO, G. V. V.; KIM, Y. J.; ORRICO, S. R. P.; CIRELLI, J. A.; MAYER, M. P. A.; et al. Periodontopathogens levels and clinical response to periodontal therapy in individuals with the interleukin-4 haplotype associated with susceptibility to chronic periodontitis. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v. 32, n. 12, p. 1501-1509, DEC 2013. (09/11371-4, 09/08773-3, 03/10424-0)

FINOTI, L. S.; CORBI, S. C. T.; ANOVAZZI, G.; TEIXEIRA, S. R. L.; STEFFENS, J. P.; SECOLIN, R.; KIM, Y. J.; ORRICO, S. R. P.; CIRELLI, J. A.; MAYER, M. P. A.; et al. Association between IL8 haplotypes and pathogen levels in chronic periodontitis. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v. 32, n. 10, p. 1333-1340, OCT 2013. (09/11371-4, 09/08773-3, 03/10424-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

FINOTI, Livia Sertori. Investigação comparativa de dados clínicos e microbiológicos entre indivíduos geneticamente suscetíveis e não suscetíveis à periodontite. 2012. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.