Proteomic analysis of adherence and biofilm formation of human sepsis-associated Escherichia coli(SEPEC)

• Grant number: 10/00587-3
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: August 01, 2010
• End date: February 28, 2014
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Tomomasa Yano
• Grantee: Rogério Arcuri Conceição
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Proteomic analysis of pathogenic bacteria, has provided numerous resources to understanding of the mechanisms by which these pathogens develop pathological processes in human. Analysis of the capacity of adhesion of sepsis-associated Escherichia coli (SEPEC) in cells of Hec-1B (endometrial adenocarcinoma human) and Vero (kidney of African green monkey), show that all strains (100%) are able to adhere and invade these cells, as well as to form biofilm in inert material. However, molecular studies of these strains indicate that SEPEC some associations between adhesins in prevalence significant, but not was possible to determine the factors involved in SEPEC adherenc. Intend, with the continuity of this work, conduct a comparative analysis of proteins expressed in terms of plancktonic cells, attached on cell surfaces and provided biofilm, so that makes it possible to identify proteins responsible the adherence of SEPEC and thus clarify its mechanisms pathogenicity.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CONCEIÇÃO, Rogério Arcuri. Proteoimics, molecular and functional analysis of potential adhesins from human sepsis associated Escherichia coli (SEPEC). 2014. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.