Intravenous glucose infusion and nutritional balance on expression of enzymes responsible for catabolism of progesterone in bovines

• Grant number: 10/01316-3
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2010
• End date: February 29, 2012
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
• Principal Investigator: José Luiz Moraes Vasconcelos
• Grantee: Fernanda Victor Rodrigues Vieira
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

The objective of this study is to evaluate the effect of intravenous glucose infusion on serum concentrations of glucose, insulin, IGF-1, progesterone (P4), mRNA expression of GHR1A, IGF-1, and mRNA expression of hepatic enzymes CYP 2C and CYP 3A responsible for the catabolism of P4 in the liver in dry cows, ovariectomized with intravaginal device P4 (CIDR) in different energy balances. Fifteen non-lactating, ovariectomized Gir × Holstein cows, and randomly assigned to: 1) negative nutrient balance (NB; n=7)) and 2) positive nutrient balance (PB; n=8). The group of cows in PB will supplement individually once a day. For the adaptation phase (d-d-28 to 15, 5), each cow will receive a CIDR of the third use, and after the adaptation phase (d-14), each cow will receive a new CIDR. On d 0, cows within nutritional treatment will be randomly assigned to receive, in a crossover design containing 2 periods of 24 h each (d0 and d1): 1) intravenous glucose infusion (0.5g/Kg of BW), or 2) intravenous saline infusion (0,9% NaCl). Immediately after fasting for 12 hours, infusions will be made over a period of three hours. Blood samples will be collected at -12 (beginning of fasting), 0 (before infusion), 3 and 6 hours after start of infusion via the coccygeal vein in vacutainer tubes without anticoagulant type. The liver biopsies will be performed at 0 and 3 hours in the day of treatment (d 0 and d 1).

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VIEIRA, Fernanda Victor Rodrigues. Infusão intravenosa de glicose e balanço energético na expressão de enzimas hepáticas responsáveis pelo catabolismo de progesterona em bovinos. 2012. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Medicina Veterinária e Zootecnia. Botucatu Botucatu.