Leucine and skeletal muscle disuse: clinical anti-atrophic efficacy and related mechanisms

• Grant number: 10/07062-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: September 01, 2010
• End date: March 31, 2013
• Field of knowledge: Health Sciences - Nutrition
• Principal Investigator: Antonio Herbert Lancha Junior
• Grantee: Humberto Nicastro Silva
• Host Institution: Escola de Educação Física e Esporte (EEFE). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The reversion/atenuation of skeletal muscle atrophy is an important framework to be investigated in several conditions able to induce it, such as immobilization-induced skeletal muscle disuse. Although skeletal muscle disuse phenomenon is widely studied, there is a lack of well controlled nutritional studies in current literature that assist the development of potential anti-atrophic strategies. This study aims to evaluate the effect of leucine supplementation isolated or combined (BCAA) on immobilization-induced (knee brace) skeletal muscle atrophy due to surgical reconstruction of anterior cruciate ligament (ACL). Male and physically active adult subjects will be randomized into 3 groups: leucine, BCAA or placebo supplementation. Both groups will be composed by subjects who underwent the procedure of ACL reconstruction. Supplementation protocol will start from 10 days prior knee surgery to 7 days after it without exercise or active rehabilitation protocol. We intend to verify the effectiveness of leucine supplementation on structure and function of quadriceps muscle and, furthermore, the possible cellular mechanisms involved in muscle remodeling and modulated by these interventions in order to propose a possible nutritional anti-atrophic strategy. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICASTRO, H.; ZANCHI, N. E.; DA LUZ, C. R.; LANCHA, JR., A. H.. Functional and morphological effects of resistance exercise on disuse-induced skeletal muscle atrophy. Brazilian Journal of Medical and Biological Research, v. 44, n. 11, p. 1070-1079, NOV 2011. (10/07062-3, 11/04690-6)

NICASTRO, HUMBERTO; DA LUZ, CLAUDIA R.; CHAVES, DANIELA F. S.; DAS NEVES, WILLIAM; VALENTE, KEROLYN S.; LANCHA, JR., ANTONIO H.. Leucine supplementation combined with resistance exercise improves the plasma lipid profile of dexamethasone-treated rats. LIPIDS IN HEALTH AND DISEASE, v. 11, JAN 11 2012. (10/07062-3, 11/04690-6)

NICASTRO, HUMBERTO; DA LUZ, CLAUDIA RIBEIRO; SEIXAS CHAVES, DANIELA FOJO; GRASSMANN BECHARA, LUIZ ROBERTO; VOLTARELLI, VANESSA AZEVEDO; ROGERO, MARCELO MACEDO; LANCHA, ANTONIO HERBERT, JR.. Does Branched-Chain Amino Acids Supplementation Modulate Skeletal Muscle Remodeling through Inflammation Modulation? Possible Mechanisms of Action. JOURNAL OF NUTRITION AND METABOLISM, v. 2012, p. 10-pg., 2012-01-01. (11/04690-6, 10/07062-3)

NICASTRO, HUMBERTO; ZANCHI, NELO EIDY; DA LUZ, CLAUDIA RIBEIRO; SEIXAS CHAVES, DANIELA FOJO; LANCHA, ANTONIO HERBERT, JR.. An Experimental Model for Resistance Exercise in Rodents. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, v. N/A, p. 7-pg., 2012-01-01. (10/07062-3, 11/04690-6)

ZANCHI, NELO EIDY; ALMEIDA, FELIPE NATALI; LIRA, FABIO SANTOS; ROSA NETO, JOSE CESAR; NICASTRO, HUMBERTO; DA LUZ, CLAUDIA RIBEIRO; DE SIQUEIRA FILHO, MARIO ALVES; FELITTI, VITOR; VAINZOF, MARIZ; SEELAENDER, MARILIA; et al. Renewed Avenues through Exercise Muscle Contractility and Inflammatory Status. SCIENTIFIC WORLD JOURNAL, 2012. (10/07062-3)

NICASTRO, HUMBERTO; ZANCHI, NELO EIDY; DA LUZ, CLAUDIA RIBEIRO; SEIXAS CHAVES, DANIELA FOJO; LANCHA, JR., ANTONIO HERBERT. An Experimental Model for Resistance Exercise in Rodents. JOURNAL OF BIOMEDICINE AND BIOTECHNOLOGY, 2012. (10/07062-3, 11/04690-6)

NICASTRO, HUMBERTO; ZANCHI, NELO E.; DA LUZ, CLAUDIA R.; DE MORAES, WILSON M. A. M.; RAMONA, PAMELLA; DE SIQUEIRA FILHO, MARIO A.; CHAVES, DANIELA F. S.; MEDEIROS, ALESSANDRA; BRUM, PATRICIA C.; DARDEVET, DOMINIQUE; et al. Effects of leucine supplementation and resistance exercise on dexamethasone-induced muscle atrophy and insulin resistance in rats. NUTRITION, v. 28, n. 4, p. 465-471, APR 2012. (10/10852-6, 10/07062-3, 11/04690-6)

ZANCHI, NELO EIDY; ALMEIDA, FELIPE NATALI; LIRA, FABIO SANTOS; ROSA NETO, JOSE CESAR; NICASTRO, HUMBERTO; DA LUZ, CLAUDIA RIBEIRO; DE SIQUEIRA FILHO, MARIO ALVES; FELITTI, VITOR; VAINZOF, MARIZ; SEELAENDER, MARILIA; et al. Renewed Avenues through Exercise Muscle Contractility and Inflammatory Status. SCIENTIFIC WORLD JOURNAL, v. N/A, p. 7-pg., 2012-01-01. (10/07062-3)