Effect of diabetes on the development of pulp infection and/or periodontal disease. Histological study in rats.

• Grant number: 10/16999-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2011
• End date: December 31, 2011
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Luciano Tavares Angelo Cintra
• Grantee: Annelise Katrine Carrara Prieto
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

This study will assess the effect of diabetes on the development of pulp and periodontal infection, singly or combined, in Wistar rats. It will be used 80 Wistar rats divided into 8 groups of 10 animals each: 1) normal rats, 2) normal rats with pulp disease, 3) normal rats with periodontal disease, 4) normal rats with pulp and periodontal diseases; 5) diabetic rats , 6) diabetic rats with pulp disease; 7) diabetic rats with periodontal disease, 8) diabetic rats with periodontal and pulp disease. Diabetes will be induced by application of alloxan and the disease development will be confirmed in the subsequent 24/48 hours by blood glucose evaluation. After confirmation of the diabetes induction, pulp and/or periodontal diseases will be induced according to the experimental group. At the end of 60 days the rats will be killed, the jaws removed and processed for laboratory evaluation in light microscopy. The tissue sections will be stained with HE and evaluated by pre-established criteria using scores to the observed microscopic events. The results will be analyzed using the Kruskall Wallis and Dunn's at a significance level of 5% (p <0.05).

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EMERENCIANO BUENO, CARLOS ROBERTO; SUMIDA, DORIS HISSAKO; HUNGARO DUARTE, MARCO ANTONIO; ORDINOLA-ZAPATA, RONALD; AZUMA, MARIANE MAFFEI; GUIMARAES, GUSTAV; PINHEIRO, TIAGO NOVAES; ANGELO CINTRA, LUCIANO TAVARES. ccuracy of radiographic pixel linear analysis in detecting bone loss in periodontal disease: Study in diabetic rat. SAUDI DENTAL JOURNAL, v. 33, n. 8, p. 987-996, DEC 2021. (10/16999-9, 11/11337-0)

ANGELO CINTRA, LUCIANO TAVARES; DA SILVA FACUNDO, AGUINALDO CANDIDO; CARRARA PRIETO, ANNELISE KATRINE; SUMIDA, DORIS HISSAKO; NARCISO, LUIS GUSTAVO; MOGAMI BOMFIM, SUELY REGINA; OLIVEIRA E SILVA, CLEVERSON; DEZAN-JUNIOR, ELOI; GOMES-FILHO, JOAO EDUARDO. Blood Profile and Histology in Oral Infections Associated with Diabetes. JOURNAL OF ENDODONTICS, v. 40, n. 8, p. 1139-1144, AUG 2014. (12/02083-8, 12/04588-0, 10/16999-9)

CINTRA, L. T. A.; SAMUEL, R. O.; FACUNDO, A. C. S.; PRIETO, A. K. C.; SUMIDA, D. H.; BOMFIM, S. R. M.; SOUZA, J. C.; DEZAN-JUNIOR, E.; GOMES-FILHO, J. E.. Relationships between oral infections and blood glucose concentrations or HbA1c levels in normal and diabetic rats. International Endodontic Journal, v. 47, n. 3, p. 228-237, MAR 2014. (12/02083-8, 10/16999-9)