Evaluation of the gastroprotective activity of chlorogenic acid in gastric ulcers induced in rats

• Grant number: 10/18069-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2011
• End date: January 31, 2012
• Field of knowledge: Health Sciences - Pharmacy - Toxicological Analysis
• Principal Investigator: Sandra Helena Poliselli Farsky
• Grantee: André Tominaga Shimoyama
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Gastric ulcer is a disease that affects a significant portion of world population. It is the result of an imbalance between aggressive and protective factors, causing injury and death of cells in the gastric tissue. There are several effective drugs used to treat this disease, but they cause serious side effects. Therefore, the search for alternative treatments is continually growing and natural products are promising agents. The chlorogenic acid (CGA) is a substance contained in a vast array of plants, food and beverages. Can be obtained by esterification reaction of hydroxycinnamic acids (p-coumaric, caffeic and ferulic) and quinic acid. Known to have antioxidant effects, anti-inflammatory, hypotensive and anti-diabetic, but the literature lacks information regarding this acid to be effective in gastric mucosal protective activity. Thus, the objective of this project is to verify the gastroprotective activity of ACG on gastric ulcers induced in rats. For this, rats are pretreated with GCA (5, 25 and 50 mg / kg, po, 1 hour before induction of ulcer) and gastric ulcer will be induced by ethanol (1.0 mL, 95%, po) or anti-inflammatory non-steroidal (indomethacin, 100 mg / kg, po). One hour after administration of ethanol or twelve hours after administration of indomethacin, the animals will be sacrificed for analysis of stomach tissue damaged area (light microscopy associated with image analysis) and for obtaining stomach tissue homogenate, which will be measured concentrations of nitric oxide (Griess reaction), prostaglandin E2 (ELISA) and tumor necrosis factor-alpha (ELISA). This project has the collaboration of Dr. Norberto Peporini Lopes, coordinator of the Laboratory for Physical Chemistry, Faculty of Pharmaceutical Sciences, University of São Paulo, Ribeirão Preto campus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SHIMOYAMA, ANDRE T.; SANTIN, JOSE ROBERTO; MACHADO, ISABEL D.; DE OLIVEIRA E SILVA, ANA MARA; PEREIRA DE MELO, ILLANA L.; MANCINI-FILHO, JORGE; FARSKY, SANDRA H. P.. Antiulcerogenic activity of chlorogenic acid in different models of gastric ulcer. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, v. 386, n. 1, p. 5-14, JAN 2013. (11/01848-8, 10/18069-9)