Anal manometric study of extrinsic denervation of anorectal segment: experimental model in rats

• Grant number: 10/18438-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2011
• End date: December 31, 2011
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Flávio Henrique Ferreira Galvão
• Grantee: Daniel Reis Waisberg
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Diseases that compromises anorectal function are frequent and its treatment still lacks improvement. Anorectal physiologic studies may contribute to the development of novel therapeutic modalities. Experimental model of anorectal dysfunction are lacking. The current project aims to evaluating the anal manometric behavior of normal rats and those undergoing extrinsic denervation of anorectal segment. Forty-five male Wistar Rats will be divided in 3 groups: 1 - anorectal extrinsic denervation, 2 - control: SHAM, 3 - control: normal rats. Surgical procedure comprises combined perineal incison and median laparotomy. We perform extraperineal dissection of distal anorectal segment and its mobilization to inside the abdominal cavity, being the inferior mesenteric vessels preserved. The cited segment will be reinserted in its perineal original site via suture between perianal and perineal skin. Group 2 will solely undergo median laparotomy and perianal incision. No surgical procedure will be performed on group 3. Anal manometry utilizing specialized device (Dynamed®: sistema DUAL®) will be performed pre-operatively, post-operatively and on 7th and 14th post-operative days on all studied groups. Weight variation and survival will be analyzed as well. On 14th post-operative day, animals will be sacrificed (study end-point). (AU)