VASP AND ZYXIN EXPRESSION AND PARTICIPATION IN HEMATOPOIETIC DIFFERENTIATION, IN CHRONIC MYELOID LEUKEMIA AND BCR-ABL SIGNALING PATHWAY

• Grant number: 11/14498-5
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: July 31, 2013
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Karin Spat Albino Barcellos Silveira
• Grantee: Vanessa Aline Bernusso
• Host Institution: Centro de Hematologia e Hemoterapia (HEMOCENTRO). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

VASP (vasodilator-stimulated phosphoprotein) and Zyxin are actin regulatory proteins that are located at cell-cell junctions. Zyxin directs actin assembly by recruiting VASP to specific sites of adhesion. The phosphorylation of VASP and Zyxin modifies its activity in cell adhesion. VASP and Zyxin act as a protein complex involved in the signal transduction for actin polymerization, in the control of cell division and in the formation and maintenance of adherent junctions. It has been described an altered expression of VASP and Zyxin in different types of tumor; however, Zyxin has not been studied in chronic myeloid leukemia (CML) and more information about VASP in CML is necessary. Thus, it is interesting to analyze the gene expression of VASP and Zyxin in K562 cell line (untreated and treated with imatinib) and in bone marrow from patients with CML. In addition, the protein expression of total and phosphorylated VASP and Zyxin will be studied in bone marrow samples of CML patients and in K652 cells. As cell adhesion proteins are important to the erythroid differentiation, especially in the nuclear extrusion, VASP and Zyxin expression will be analyzed during erythroid differentiation and the effect of VASP and Zyxin inhibition in the erythroid differenciation will be evaluated.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERNUSSO, VANESSA A.; MACHADO-NETO, JOAO A.; PERICOLE, FERNANDO V.; VIEIRA, KARLA P.; DUARTE, ADRIANA S. S.; TRAINA, FABIOLA; HANSEN, MARC D.; SAAD, SARA T. OLALLA; BARCELLOS, KARIN S. A.. Imatinib restores VASP activity and its interaction with Zyxin in BCR-ABL leukemic cells. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1853, n. 2, p. 388-395, FEB 2015. (08/57895-1, 11/51959-0, 11/14498-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BERNUSSO, Vanessa Aline. VASP and Zyxin expression and participation in hematopoietic differentiation, in chronic myeloid leukemia and BCR-ABL signaling pathway. 2013. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.