Fluorescence Imaging using variable liquid crystal tunable filter for citrus diseases

• Grant number: 11/22275-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: March 01, 2012
• End date: December 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Physics - Atomic and Molecular Physics
• Principal Investigator: Luis Gustavo Marcassa
• Grantee: Caio Bruno Wetterich
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated scholarship(s): 13/11023-1 - Use o fluorescence images for a comparative study of diseases in citrus samples from Florida and São Paulo, BE.EP.DR

Abstract

Over the past eight years, our laboratory has developed in conjunction with a project Fundecitrus application of photonic technology to detect diseases in citrus. In this PhD project will build a fluorescence imaging system using high power LEDs and a highly monochromatic liquid crystal optical filter variable. The filter variable will allow us to scan images of fluorescence from 450 to 740 nm. The images are analyzed using PCA (Principal Component Analysis) and SVM (Support vector machine). In addition, we study polarized fluorescence images. These techniques allow us to identify if there are bands of fluorescence specific for each disease. Having seen the results of the previous project in the limit the following diseases: citrus canker, scab, Greening, Rubelose buds and, in addition to zinc deficiency.

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WETTERICH, CAIO BRUNO; DE OLIVEIRA NEVES, RUAN FELIPE; BELASQUE, JOSE; MARCASSA, LUIS GUSTAVO. Detection of citrus canker and Huanglongbing using fluorescence imaging spectroscopy and support vector machine technique. APPLIED OPTICS, v. 55, n. 2, p. 400-407, JAN 10 2016. (10/16536-9, 11/22275-6)

WETTERICH, CAIO BRUNO; DE OLIVEIRA NEVES, RUAN FELIPE; BELASQUE, JOSE; EHSANI, REZA; MARCASSA, LUIS GUSTAVO. Detection of Huanglongbing in Florida using fluorescence imaging spectroscopy and machine-learning methods. APPLIED OPTICS, v. 56, n. 1, p. 9-pg., 2017-01-01. (11/22275-6, 13/11023-1, 10/16536-9)

WETTERICH, CAIO B.; LINS, EMERY C.; BELASQUE, JR., JOSE; MARCASSA, LUIS G.. Observation of Climacteric-Like Behavior of Citrus Leaves Using Fluorescence Spectroscopy. JOURNAL OF SPECTROSCOPY, p. 1-6, 2014. (10/16536-9, 11/22275-6)

WETTERICH, CAIO BRUNO; DE OLIVEIRA NEVES, RUAN FELIPE; BELASQUE, JOSE; EHSANI, REZA; MARCASSA, LUIS GUSTAVO. Detection of Huanglongbing in Florida using fluorescence imaging spectroscopy and machine-learning methods. APPLIED OPTICS, v. 56, n. 1, p. 15-23, JAN 1 2017. (11/22275-6, 10/16536-9, 13/11023-1)

WETTERICH, CAIO B.; LINS, EMERY C.; BELASQUE, JOSE, JR.; MARCASSA, LUIS G.. Observation of Climacteric-Like Behavior of Citrus Leaves Using Fluorescence Spectroscopy. JOURNAL OF SPECTROSCOPY, v. 2014, p. 6-pg., 2014-01-01. (11/22275-6, 10/16536-9)

NEVES, RUAN F. O.; WETTERICH, CAIO B.; SOUSA, ELAINE P. M.; MARCASSA, LUIS G.. Multiclass classifier based on deep learning for detection of citrus disease using fluorescence imaging spectroscopy. Laser Physics, v. 33, n. 5, p. 9-pg., 2023-05-01. (11/22275-6, 13/11023-1, 10/16536-9)

WETTERICH, CAIO BRUNO; DE OLIVEIRA NEVES, RUAN FELIPE; BELASQUE, JOSE; MARCASSA, LUIS GUSTAVO. Detection of citrus canker and Huanglongbing using fluorescence imaging spectroscopy and support vector machine technique. APPLIED OPTICS, v. 55, n. 2, p. 8-pg., 2016-01-10. (11/22275-6, 10/16536-9)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

WETTERICH, Caio Bruno. Fluorescence image applied on citrus diseases. 2016. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Física de São Carlos (IFSC/BT) São Carlos.