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Phenotipic, genetic and pathogenic characterization of Burkholderia andropogonis strains from different host plants

Grant number: 11/12222-2
Support Opportunities:Scholarships in Brazil - Doctorate
Start date: January 01, 2012
End date: July 31, 2015
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal Investigator:Suzete Aparecida Lanza Destéfano
Grantee:Lucilene Lopes dos Santos
Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

This project will aim the study of 23 strains of Burkholderia andropogonis, important pathogen of 52 species from 15 families of plants economically important to the country as corn, sorghum, coffee, and ornamental plants like orchids, carnation among others, with wide geographical distribution. Other strains of this bacterial species will be acquired in international collections and included in this study in order to enlarge the host range. Due the complexity and pathogenic specialization and the few information about of the factors that determine the genetic diversity and pathogenic of the B. andropogonis, it is very important the differentiation of these strains, and polyphasic taxonomy studies may help in elucidating the infra-specific diversity, promoting understanding of the mechanisms involved in the interaction of bacteria with different hosts. The phenotypic and molecular characterization will be carried out with different genetic markers as 23SDNAr-16S spacer region, genes gyrB, rpoD and rpoB, and analysis of genetic diversity of strains by rep-PCR technique (BOX, ERIC and REP-PCR), and evaluation of pathogenicity in different groups of host plants by artificial inoculation on different hosts.

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LOPES-SANTOS, LUCILENE; ASSUMPCAO CASTRO, DANIEL BEDO; FERREIRA-TONIN, MARIANA; ALVES CORREA, DANIELE BUSSIOLI; WEIR, BEVAN SIMON; PARK, DUCKCHUL; MARISCAL OTTOBONI, LAURA MARIA; RODRIGUES NETO, JULIO; LANZA DESTEFANO, SUZETE APARECIDA. Reassessment of the taxonomic position of Burkholderia andropogonis and description of Robbsia andropogonis gen. nov., comb. nov.. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, v. 110, n. 6, p. 727-736, . (11/12222-2)
LOPES-SANTOS, LUCILENE; ASSUMPCAO CASTRO, DANIEL BEDO; MARISCAL OTTOBONI, LAURA MARIA; PARK, DUCKCHUL; WEIR, BEVAN SIMON; LANZA DESTEFANO, SUZETE APARECIDA. Draft Genome Sequence of Burkholderia andropogonis Type Strain ICMP2807, Isolated from Sorghum bicolor. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 3, n. 3, . (11/12222-2)
LOPES-SANTOS, LUCILENE; ASSUMPCAO CASTRO, DANIEL BEDO; MARISCAL OTTOBONI, LAURA MARIA; PARK, DUCKCHUL; WEIR, BEVAN SIMON; LANZA DESTEFANO, SUZETE APARECIDA. Draft Genome Sequence of Burkholderia andropogonis Type Strain ICMP2807, Isolated from Sorghum bicolor. GENOME ANNOUNCEMENTS, v. 3, n. 3, p. 2-pg., . (11/12222-2)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
SANTOS, Lucilene Lopes dos. Biochemical, genetic and pathogenic characterization of Burkholderia andropogonis and taxonomic reassessment of the species. 2015. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.