Effects of hydrogen peroxide in the medial septal area on the water intake, urinary excretion and pressor response induced by cholinergic stimulation of the medial septal area

• Grant number: 11/15340-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2012
• End date: December 31, 2013
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Eduardo Colombari
• Grantee: Mariana Del Rosso de Melo
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The medial septal area (MSA) is a forebrain area involved in cardiovascular control and fluid-electrolytic balance. This area is rich in cholinergic receptors, and the activation of this area induces thirst, natriuresis, antidiuresis and an increase the arterial pressure. Experimental evidence shows that reactive oxygen species (ROS) may participate of control physiological responses. Recent results of our laboratory demonstrated that a reactive oxygen species, the hydrogen peroxide (H2O2), injected into lateral ventricle (LV) decrease the water intake induced by ANG II and carbachol (cholinergic agonist) and decreases the pressor response induced by ANG II also injected into LV. Preliminary results showed that the dipsogenic, antidiuretic and natriuretic resoonse of carbachol into MSA was decreased by previous injection of H2O2 (5 umol/0,5 uL) in also MSA. However, this dose of H2O2 reduced sucrose solution intake (2%), suggesting that at this dose H2O2 may have unspecific effects. Thus, in the present project, we propose to study the effects of lower doses (0,1, 1,0 e 2,5 umol/0,5 uL) of H2O2 in the MSA on water intake, renal excretion of water and electrolytes and pressor response induced by cholinergic stimulation of MSA; 2) to analyze the effects of lower doses (0,1, 1,0 e 2,5 umol/0,5 uL) of H2O2 on food and sucrose 2% intakes; 3) to verify the effect on c-Fos expression in hypothalamus (PVN and SON) after the carbachol injection into MSA and verify whether the H2O2 into MSA changes this expression. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MELO, M. R.; MENANI, J. V.; COLOMBARI, E.; COLOMBARI, D. S. A.. HYDROGEN PEROXIDE ATTENUATES THE DIPSOGENIC, RENAL AND PRESSOR RESPONSES INDUCED BY CHOLINERGIC ACTIVATION OF THE MEDIAL SEPTAL AREA. Neuroscience, v. 284, p. 611-621, JAN 22 2015. (11/15340-6)