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Adosterone effect on the expression of heat shock proteins (hsps) in immortalized rat proximal tubule cells (IRPTC)

Grant number: 11/23757-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2012
Effective date (End): March 31, 2013
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal researcher:Deise Carla Almeida Leite Dellova
Grantee:Lillian Gonzalez Bonifacio
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil


Recently, our publications demonstrate nongenomic effects of aldosterone in proximal tubule via glucocorticoid receptor (GR), however the participation of this receptor in the genomic effects of aldosterone in the proximal tubule has not been studied. The GR is present in the cell citoplasm acoupled with chaperone proteins, called heat shock proteins (hsps), and the hsp 90-KDa (hsp 90) is considered the most important by some authors. After hormone binding to the GR, occurs the hsps dissociation of the receptor and studies suggest that hsps free in the cell citoplasm have an important biological role, as the hormone signaling. The objective of the present project is study the adosterone effect on the expression of hsps in cell line of rat proximal tubule, order to clarify the GR involvement and suggest the participation of hsp 90 in the signaling pathway of aldosterone genomic effects in the proximal tubule. For the implementation of this project will use the immortalized rat proximal tubule cells (IRPTC) as experimental model (since it does not have mineralocorticoid receptor (MR) but abundantly expressed GR) and RT-PCR (Reverse Transcriptase - Polymerase Chain Reaction).